20.500.12556/DKUM-96776 The effect of combined elicitation with light and temperature on the chlorogenic acid content, total phenolic content and antioxidant activity of berula erecta in tissue culture Chlorogenic acid is one of the most prominent bioactive phenolic acids with great pharmacological, cosmetic and nutritional value. The potential of Berula erecta in tissue culture was investigated for the production of chlorogenic acid and its elicitation combined with light of different wavelengths and low temperature. The content of chlorogenic acid in the samples was determined by HPLC-UV, while the content of total phenolic compounds and the antioxidant activity of their ethanol extracts were evaluated spectrophotometrically. The highest fresh and dry biomasses were obtained in plants grown at 23 °C. This is the first study in which chlorogenic acid has been identified and quantified in Berula erecta. The highest chlorogenic acid content was 4.049 mg/g DW. It was determined in a culture grown for 28 days after the beginning of the experiment at 12 °C and under blue light. The latter also contained the highest content of total phenolic compounds, and its extracts showed the highest antioxidant activity. Berula erecta could, potentially, be suitable for the in vitro production of chlorogenic acid, although many other studies should be conducted before implementation on an industrial scale. Berula erecta Apiaceae plant tissue culture elicitation phenolic compounds chlorogenic acid antioxidant activity ozkolistni koščec kobulnice rastlinska tkiva izvabljanje fenoli klorogenska kislina antioksidativno delovanje true false true MDPI Angleški jezik Slovenski jezik https://www.mdpi.com/journal/plants © 2024 by the authors Članek v reviji 2026-01-28 12:54:31 2026-01-28 12:54:31 2026-01-29 03:11:47 0000-00-00 00:00:00 2024 0 0 20 str. iss. 11, [article no.] 1463 Vol. 13 May 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2024-03-14 2024-05-23 2024-05-25 66.02 197046019 10.3390/plants13111463 2223-7747 plants-13-01463.pdf plants-13-01463.pdf 1 479F7DE4A9BEA16ADBD53AB08701D6D4 aa1d04aa7e9363397cc99e7cb59e0457da354617f0920769feedff4b175afeff 6bb7a7aa-fc40-11f0-9a72-00155d00018d https://dk.um.si/Dokument.php?lang=slv&id=202687 https://www.mdpi.com/2223-7747/13/11/1463 0 64a9ac5a-fc40-11f0-9a72-00155d00018d https://dk.um.si/Dokument.php?lang=slv&id=202686 Fakulteta za kemijo in kemijsko tehnologijo Fakulteta za kmetijstvo in biosistemske vede Fakulteta za naravoslovje in matematiko Pedagoška fakulteta 0 0 0