20.500.12556/DKUM-97031 P. lanceolata L. and P. major L. extracts in 3D-printed polysaccharide dressings for improved wound healing in vitro Plantago species have long been used in traditional medicine for wound healing, yet systematic validation remains limited. To explore their potential, we extracted active compounds from Plantago major (PM) and Plantago lanceolata (PL) using various solvents and incorporated them into a novel wound care formulation. The PM extract contained primarily plantamajoside, followed by verbascoside and aucubin, while the PL extract was rich in verbascoside, aucubin, and catalpol. Extract safety was evaluated on skin fibroblasts and keratinocytes using an MTT assay. PM extract (1000.0 μg/mL) increased fibroblast metabolic activity to 1.2 without cytotoxicity toward keratinocytes, while PL extract (100.0 μg/mL) enhanced keratinocyte viability to 1.3 without significant fibroblast effects. Methanol and ethanol extracts of PM and PL were incorporated into a polysaccharide-based wound dressing (WD) composed of methylcellulose, alginate, and nanofibrillated cellulose. The WDs were comprehensively characterized for swelling, degradation, and active compound release, as well as structural features using scanning electron microscopy, nanotomography, and atomic force microscopy. Mechanical strength and adhesion testing confirmed suitable handling and application properties, while anti-inflammatory assays demonstrated additional therapeutic potential of the extracts. Biocompatibility testing showed that the incorporation of 0.5 wt% extract increased fibroblast viability to 1.39 for PM WD and 1.5 for PL WD. Scratch assays on fibroblasts demonstrated enhanced wound closure, with PM- and PL-infused WDs closing wounds by 64% ± 7% and 52% ± 7%, respectively, compared to 29% ± 6% for the control and 33% ± 11% for the WD without extract. These results highlight the multifunctional potential of PM and PL extracts in wound healing applications. Plantago bioactive compounds 3D printing release kinetics pharmacology of wound healing bioaktivne spojine 3D tiskanje kinetika sproščanja farmakologija celjenja ran true false true Elsevier Angleški jezik Slovenski jezik https://www.sciencedirect.com/science/article/pii/S0141813026004629?via%3Dihub Članek v reviji 2026-02-12 10:20:30 2026-02-12 10:20:31 2026-04-20 08:00:54 0000-00-00 00:00:00 2025 0 0 22 str. Vol. 344 [article no.] 150536 Feb. 2026 0000-00-00 Zaloznikova Objavljeno apc 2025-04-09 2026-01-26 2026-01-29 577:615 266695683 10.1016/j.ijbiomac.2026.150536 1879-0003 1-s2.0-S0141813026004629-main.pdf 1-s2.0-S0141813026004629-main.pdf 1 194D013D576676DF9733C1E085769A41 7fa47fdd8edcbdaa7440f1fc650cc32d25fb241600e314c8b7659e580044581f 1f5dc24f-080c-11f1-93cb-00155d00019a https://dk.um.si/Dokument.php?lang=slv&id=203268 https://www.sciencedirect.com/science/article/pii/S0141813026004629?via%3Dihub 0 2da034ec-080c-11f1-93cb-00155d00019a https://dk.um.si/Dokument.php?lang=slv&id=203269 Fakulteta za strojništvo Fakulteta za kemijo in kemijsko tehnologijo Medicinska fakulteta 0 0 0