20.500.12556/DKUM-97032 Solid-state platform (SSP) to produce terpenes from enzymatically treated textile waste with E. coli Recycling waste has become one of the critical issues facing modern societies, with textile waste streams serving as a primary example for the recycling of materials made from cotton, PET, other plastics, and their mixtures. One method that has gained traction in recent years is enzymatic hydrolysis of textile waste and the subsequent conversion of cotton to glucose. Glucose serves as the carbon source for engineered microbes that convert it to volatile value-added chemicals such as terpenes. In this work, we present a novel strategy to produce volatile organic compounds (VOCs), the ‘Solid-State Platform’ (SSP) for the microbial synthesis of terpenes. This platform offers a sustainable approach for utilising enzymatically treated cotton-based waste and its efficient transformation by engineered Escherichia coli into VOCs, which are trapped directly as vapour by the organic overlay, dodecane, present within the SSP. The engineered microbial system is constructed from conventional inert laboratory glass, without gaskets, filters, or high-tech custom-made equipment. Using pure glucose as the carbon source 1.4 mg mL−1 of limonene and 0.7 mg mL−1 cineole was produced respectively using the SSP. Using enzymatically treated waste materials, specificaly glucose juice H46, 0.35 mg mL−1 of cineole was produced by SSP. The SSP represents an alternative strategy for producing green volatile value-added chemicals and may help mitigate the environmental burden of textile disposal by broadening the assortment of recycling technologies. textile waste recycling E.coli tekstilni odpadki recikliranje bakterije E.coli analizne metode true false true Royal society of chemistry Angleški jezik Slovenski jezik https://pubs.rsc.org/en/content/articlelanding/2026/ra/d5ra06351e Članek v reviji 2026-02-12 13:24:46 2026-02-12 13:24:47 2026-02-13 03:12:13 0000-00-00 00:00:00 2025 0 0 str. 3163-3168 Vol. 16 iss. 4 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2025-08-26 2025-12-22 2026-01-14 677.017:543.2 267196419 10.1039/d5ra06351e 2046-2069 d5ra06351e.pdf d5ra06351e.pdf 1 E50E1DA0BBB958EBDB37645DF9298448 eef5fa7c9a446240e225ce0d44cec8f8e81c66fa03e8794a5bd20cb3acf76c70 88e2e2d9-080e-11f1-93cb-00155d00019a https://dk.um.si/Dokument.php?lang=slv&id=203270 https://pubs.rsc.org/en/content/articlelanding/2026/ra/d5ra06351e 0 b9025f0c-080e-11f1-93cb-00155d00019a https://dk.um.si/Dokument.php?lang=slv&id=203271 Fakulteta za strojništvo 0 0 0