20.500.12556/DKUM-97110 Biocompatibility of Supercritical Carbon Dioxide Processed Poly(Lactide-Co-Glycolide) and Poly(ε-Caprolactone) Assessed With Primary Human Osteoblasts Biokompatibilnost z metodo superkritičnega ogljikovega dioksida procesiranih poli(laktid-ko-glikolida) in poli([epsilon]-kaprolaktona) na primarnih človeških osteoblastih Purpose: In the present study, poly (lactide-co-glycolide) (PLGA) and poly (ε-caprolactone) (PCL) combined with hydroxyapatite (HA) were processed with supercritical CO2 (scCO2) to obtain macroporous foams. Methods: The in vitro biocompatibility and mineralization of the foams was assessed with primary human osteoblasts (HOB). Results: The WST-8 (Water-soluble Tetrazolium salts) test for cell proliferation activity and alkaline phosphatase (ALP) activity assay were used to estimate HOB proliferation and differentiation respectively. The proliferation activity of HOB on foamed PLGA and PCL decreased compared to non-foamed polymer, while on PLGA and PCL foamed constructs with hydroxyapatite (HA), the proliferation activity increased compared to the foamed PLGA and PCL. Conclusions: The ALP activity on PLGA, PCL and foamed PCL was similar to the control, being less intense on PCL foamed constructs with HA, while foamed PLGA and PLGA foamed constructs with HA showed no evidence of ALP activity. Namen: V študiji smo preverjali biokompatibilnost poli(laktid- -ko-glikolid) (PLGA) in poli(ε- kaprolakton) (PCL) na primarnih človeških osteoblastih (HOB). Metode: Za preizkušanje proliferacijske aktivnosti celic smo uporabili test WST-8 (Water-soluble Tetrazolium salts). Stopnjo diferenciacije HOB smo ocenjevali s testom aktivnosti alkalne fosfataze. Rezultati: Proliferacijska aktivnost HOB je bila na penah PLGA in PCL zmanjšana v primerjavi s PLGA in PCL, na konstruktih pen PLGA in PCL s hidroksiapatitom (HA) pa zvečana glede na proliferacijsko aktivnost celic na penah PLGA in PCL. Zaključek: Aktivnost alkalne fosfataze na PLGA, PCL in peni PCL se ni razlikovala od kontrole. Na konstruktih pen PCL s HA je bila aktivnost alkalne fosfataze manjša v primerjavi s kontrolo, medtem ko se na peni PLGA in konstruktih pen PLGA s HA aktivnost alkalne fosfataze ni izrazila. biocompatibility supercritical carbon dioxide foaming poly(lactide-co-glycolide) poly([epsilon]- caprolactone) primary human osteoblasts biokompatibilnost penjenje s superkritičnim ogljikovim dioksidom poli(laktid-ko-glikolid) poli([epsilon]-kaprolakton) primarni človeški osteoblasti true false true Angleški jezik Slovenski jezik Znanstveno delo 2026-02-18 13:31:30 2026-02-18 13:31:30 2026-03-19 11:42:32 0000-00-00 00:00:00 2016 0 Maribor 0 7 št. 1 Letn. 9 2016 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2015-09-18 2016-02-01 2016-01-01 61 512624184 10.18690/actabiomed.169 1855-5640 RAZ_Daris_Barbara_2016.pdf RAZ_Daris_Barbara_2016.pdf 1 C22E694A97EF158062ECB20A34ED9597 cb1b3b683fe41ef67622aaca4dc395b2ca863a19ab3e21fa334f5a3f80d06ed0 9d53b774-0cc7-11f1-8b1a-00155d580e1a https://dk.um.si/Dokument.php?lang=slv&id=203463 https://journals.um.si/index.php/amb/article/view/1537 0 c295cf2a-0cc5-11f1-8b1a-00155d580e1a https://dk.um.si/Dokument.php?lang=slv&id=203461 Medicinska fakulteta Acta medico-biotechnica 0 0 0