20.500.12556/DKUM-97212 Izraba tekočih farmacevtskih odpadkov za proizvodnjo biogoriva magistrsko delo Utilization of liquid pharmaceutical waste for biofuel production V magistrskem delu smo izvedli anaerobno mono-digestijo odpadnega farmacevtskega micelija (MIC) ter različnih komunalnih in industrijskih odpadkov, kot so anaerobni mulj (M), lanena pogača (LP), rožmarinove tropine (RT), neobdelana konopljina pogača (NKP) in konopljina pogača obdelana z glivo Thermomyces lanuginosus (OKP). Izvedli smo tudi so-digestijo posameznih substratov z dodatkom micelija (M+MIC, LP+MIC, RT+MIC, OKP+MIC, NKP+MIC) ter so-digestijo mešanice izbranih substratov (M+LP+RT+MIC). Vzorce smo inokulirali s prašičjo gnojevko. Proces je potekal pri mezofilnih pogojih (38 °C), 27 dni, kjer smo vsakodnevno spremljali količino in sestavo nastalega bioplina. Izkoristek bioplina smo izrazili kot volumen proizvedenega bioplina (mL) na gram hlapne snovi (angl. volatile solid, VS). Med procesom smo analizirali tekoče digestate, pri čemer smo spremljali ključne kemijske parametre. Po koncu eksperimenta smo s testom sprejemljivosti za rastline ocenili potencial digestatov kot izboljševalcev tal. Najvišji izkoristek bioplina pri mono-digestiji, 281 mL/gVS, smo dosegli pri vzorcu NKP. Sledijo vzorci RT (267 mL/gVS), M (256 mL/gVS), OKP (190 mL/gVS) in LP (160 mL/gVS). Celokupni volumen bioplina pri MIC je bil 77 mL/gVS. Najvišji izplen bioplina pri so-digestiji smo izmerili vzorcu M+LP+RT+MIC (573 mL/gVS), sledijo RT+MIC (488 mL/gVS), M+MIC (389 mL/gVS), OKP+MIC (170 mL/gVS) in NKP+MIC (134 mL/gVS) ter LP+MIC (63 mL/gVS). Dodatek micelija je izboljšal izplen bioplina pri substratu M in RT, pri ostalih vzorcih je proizvodnjo bioplina celo znižal. Najvišjo vsebnost metana (69,2 vol. %) in najvišjo kalorično vrednost bioplina (26,7 MJ/kg) smo pri mono-digestiji izmerili vzorcu RT. Pri so-digestiji pa pri vzorcu M+MIC, kjer je bila vsebnost metana 76,9 vol. % in kalorična vrednost 31,8 MJ/kg. Učinkovitost odstranjevanja kemijske potrebe po kisiku se je gibala med 14 % in 65 % za mono-digestijo in med 28 % in 56 % za so-digestijo. V vseh reakcijskih mešanicah smo izmerili razmeroma visoke koncentracije maščobnih kislin in amonijevih ionov. Kinetična analiza mono-digestije je pokazala najboljše ujemanje eksperimentalnih podatkov z modificiranim Gompertz-ovim modelom (R² > 0,99), sledila sta kinetični model 1. reda in Monodov model (R2 >0,98) ter logistični kinetični model (R2 >0,97). Enako smo pri so-digestiji najboljše ujemanje dobili pri modificiranem Gompertz-ovem modelu (R² > 0,96), čeprav je bilo prileganje slabše kot pri mono-digestiji. Digestati so-digestije so bili pri testu sprejemljivosti za rastline manj uspešni kot digestati mono-digestije. Po končani anaerobni digestiji smo izbrane vzorce izpostavili procesu hidrotermalne karbonizacije (HTC), z namenom, da bi pridobili hidro-oglje. Vzorcu LP+MIC smo izmerili najvišjo kurilno vrednost hidro-oglja, 28,2 MJ/kg. Tekoče produkte HTC smo kemijsko ovrednotili in jih izpostavili testu sprejemljivosti za rastline, kjer smo ugotovili, da je za potrebe izboljšanja tal primerna procesna tekočina vzorca M+LP+RT+MIC. In the master's thesis, we conducted anaerobic mono-digestion of waste pharmaceutical mycelium (MIC) and various municipal and industrial wastes, including sewage sludge (M), flaxseed cake (LP), rosemary pomace (RT), untreated hempseed cake (NKP) and hempseed cake pretreated with the fungus Thermomyces lanuginosus (OKP). Additionally, we carried out co-digestion of individual substrates with mycelium (M+MIC, LP+MIC, RT+MIC, OKP+MIC, NKP+MIC) and co-digestion of a mixture of selected substrates (M+LP+RT+MIC). The samples were inoculated with pig slurry. The process was conducted under mesophilic conditions (38 °C), with a hydraulic retention time of 27 days, and daily monitoring of biogas production and composition. Biogas yield was expressed as the volume (mL) of biogas produced per gram of volatile solid (VS). During the process, we analysed the liquid digestates by monitoring key chemical parameters. After the experiment, we assessed their potential use as soil improvers by performing a plant growth test. The highest biogas yield in the mono-digestion test, 281 mL/gVS, was obtained with the NKP sample, followed by RT (267 mL/gVS), M (256 mL/gVS), OKP (190 mL/gVS), and LP (160 mL/gVS) samples. The total biogas volume in the MIC sample was 77 mL/gVS. The highest biogas yield in the co-digestion test was obtained with the M+LP+RT+MIC sample (573 mL/gVS), followed by RT+MIC (488 mL/gVS), M+MIC (389 mL/gVS), OKP+MIC (170 mL/gVS), NKP+MIC (134 mL/gVS), and LP+MIC (63 mL/gVS) samples. The addition of mycelium increased biogas yield in substrates M and RT, whereas in the other samples it caused a decline in biogas production. The highest methane content (69.2 vol.%) and the highest calorific value of biogas (26.7 MJ/kg) in mono-digestion were measured in the RT sample. In co-digestion, the highest methane content (76.9 vol.%) and the highest calorific value (31.8 MJ/kg) were obtained in the M+MIC sample. The chemical oxygen demand removal efficiency ranged from 14% to 65% for mono-digestion and from 28% to 56% for co-digestion. High fatty acids content and ammonium ion concentrations were measured in all reaction mixtures. Kinetic analysis of the mono-digestion showed that the modified Gompertz kinetic model provided the best fit to the experimental data (R² > 0.99), followed by the First order kinetic model and the Monod model (R² > 0.98), and the logistic kinetic model (R2 >0.97). Similarly, in the co-digestion experiments, the modified Gompertz model again achieved the best fit (R² > 0.96), although the model fit was reduced compared with mono-digestion. The digestates from co-digestion performed less effectively in the cress seeds germination test compared with the mono-digestion digestates. After anaerobic digestion, selected samples were subjected to hydrothermal carbonization (HTC) to produce hydrochar. The highest calorific value of hydrochar, 28.2 MJ/kg, was measured for the LP+MIC sample. The liquid products of HTC were chemically characterised and evaluated in cress seeds growth tests, where the process liquid of the M+LP+RT+MIC sample proved suitable for application as a soil amendment. odpadni farmacevtski micelij anaerobna so-digestija proizvodnja bioplina valorizacija digestata kinetična analiza hidrotermalna karbonizacija pharmaceutical mycelial residue anaerobic co-digestion biogas production digestate valorisation kinetic analysis hydrothermal carbonization true true false [A. Rajh] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-02-23 21:11:08 2026-03-25 13:34:32 2026-03-28 03:17:10 0000-00-00 00:00:00 2026 0 Maribor 0 Maribor 1 spletni vir (1 datoteka PDF (IV, 60 str.)) 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.098.4:[628.4.038:615.012](043.2) 273376771 MAG_Rajh_Ana_2026.pdf MAG_Rajh_Ana_2026.pdf 1 FCBB4951E552735D8927B0084E18489E 91ed4dc871ce239cb2404113d9c09bcefb9c143d26ee24148aff158a4daa9469 f1a7812c-275d-11f1-bb13-00155d0001a1 https://dk.um.si/Dokument.php?lang=slv&id=204740 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0