20.500.12556/DKUM-97589 Sustainable extraction of chitosan from blue crabs (Callinectes sapidus) by green solvents Chitosan, one of the significant marine polysaccharides, has attracted widespread attention due to its excellent biocompatibility and broad application prospects. This study proposes a novel green extraction method based on Brønsted acid-base deep eutectic solvents (DESs) for the efficient production of chitosan from the exoskeleton of blue crabs, achieving a green alternative to traditional inorganic acid-base chemical processes. Unlike conventional processes, this study innovatively replaces inorganic acids with acidic DESs and sodium hydroxide with alkaline DES, establishing an environmentally friendly deacetylation route. The effects of four different acidic DESs on chitosan purity were systematically compared. The produced chitosan was characterized by Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and nuclear magnetic resonance (1H NMR). Characteristic NMR peaks at 4.6 and 4.8 ppm confirm the formation of glucosamine structures, indicating that the purity of the resulting chitosan is comparable to that of commercial one. Furthermore, this study established a process technology roadmap and life cycle assessment (LCA) model to identify environmental impact hotspots during the extraction process. The results showed that electricity consumption during the manufacturing phase was the primary source of environmental burden, providing clear guidance for subsequent energy optimization and industrial scale-up. chitosan carbon reduction deep eutectic solvents sustainability life cycle assessment citosan redukcija ogljika življenjski cikel true false true Elsevier Ltd. Angleški jezik Slovenski jezik https://www.sciencedirect.com/journal/bioresource-technology Članek v reviji 2026-03-23 13:43:58 2026-03-23 13:43:59 2026-03-24 03:12:17 0000-00-00 00:00:00 2026 0 0 12 str. Vol. 441, [article no.] 133595 Feb. 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2025-04-18 2025-11-03 2025-11-04 66 272304387 10.1016/j.biortech.2025.133595 1873-2976 1-s2.0-S0960852425015627-main.pdf 1-s2.0-S0960852425015627-main.pdf 1 2644E017332F86CDD68EC7D3BD01911E 662980cde3108402f52cb2c66b005327e27a73cdbc67e2d063748234d8885c7f 340aed3e-26b6-11f1-ab09-00155d0001a1 https://dk.um.si/Dokument.php?lang=slv&id=204716 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0