20.500.12556/DKUM-9810 POMEN DOLOČEVANJA RETIKULOCITNEGA EKVIVALENTA HEMOGLOBINA PRI BOLNIKIH Z ANEMIJO MEANING OF DETERMINATION OF RETICULOCYTE HEMOGLOBIN EQUIVALENT IN THE DIAGNOSIS OF ANEMIA Namen diplomske naloge je bil določitev novega hematološkega parametra RET He (retikulocitni ekvivalent hemoglobina) s pretočno citometrijo pri treh skupinah bolnikov z anemijo: bolnikih z anemijo zaradi pomanjkanja železa, bolnikih z anemijo zaradi pomanjkanja železa, ki so se zdravili s preparati železa in bolnikih z okvaro kostnega mozga (mielodisplastični sindrom), ki so anemični, ker kostni mozeg ne proizvaja dovolj rdečih krvnih celic ali pa so te nefunkcionalne (displastični). Dobljene vrednosti smo primerjali s klasičnimi hematološkimi (Hb, MCH, MCV, MCHC) in biokemijskimi (serumsko železo, feritin) kazalci pomanjkanja železa. Pri bolnikih z anemijo zaradi pomanjkanja železa so rezultati pokazali visoko korelacijo med RET He in hematološkimi parametri (r=0,83-0,92). Med RET He in feritinom je nekoliko nižja (0,73). Najnižja je med RET He in serumskim železom (0,65). Pri bolnikih z MDS je zaradi kompleksnosti bolezni vrednost parametra RET He težje opredeljiva. The purpose of my diploma thesis was to determine the new hematological parameter of RET He (reticulocyte hemoglobin equivalent) with the help of the flow cytometry. We determined it at three groups of anemic patients: patients with iron deficiency anemia, patients with iron deficiency anemia who were healed by iron, and the patients who have damaged bone marrow (myelodysplastic syndrome) and are anemic because the bone marrow does not produce enough red blood cells or the cells are dysfunctional. Gained values were compared to classical hematological (Hb, MCH, MCV, MCHC) and biochemical (serum iron and feritin) indicators of iron deficiency. Results at patients with iron deficiency anemia showed high correlation between med RET He and hematological parameters (r=0,83-0,92). Correlation between RET He and feritin is lower (0,73). The lowest is between RET He and serum iron (0, 65). At MDS patients the value of the parameter is hard to define because of complexity of the illness. kri anemija metabolizem železa mielodisplastični sindrom retikulocitni ekvivalent hemoglobina blood anemia iron metabolism myelodysplastic sydrome reticulocyte hemoglobin equivalent true true false [M. Lesjak] Slovenski jezik Angleški jezik Diplomsko delo 2009-02-18 10:55:42 2009-03-02 09:44:25 2022-04-11 15:46:23 2022-04-15 03:23:09 2009 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 611.018.1:615.155.16(043.3) 13036822 URN:SI:UM:DK:YXVT9NVH UNI_Lesjak_Marija_2009.pdf UNI_Lesjak_Marija_2009.pdf 1 830B4578E036EA0A407237633CA4D648 d8f2e7c338f117c1a19132f2c21f94778d438d7f016bdfb492fe69defd7638f8 a0e173db-7c02-11eb-bb7a-00155d0001ca 20.500.12556/dkum/bad5712a-f2a2-4fb6-8953-28ff77621ff1 https://dk.um.si/Dokument.php?lang=slv&id=7431 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0