20.500.12556/DKUM-9843 Primerjava odpornosti na mraz trogloksenih in troglofilih vrst med zimsko in poletno diapavzo Comparison of trogloxene and troglophile species to cold-hardiness during winter and summer diapause Odpornost na mraz je sposobnost organizmov, da preživijo krajšo ali daljšo izpostavitev nizkim temperaturam, ki so nižje od temperature zmrzovanja njihovih telesnih tekočin. To odpornost lahko izmerimo z določitvijo temperature smrtnosti zaradi zmrznjenja oziroma točke zmrznitve, LT50, pri kateri pogine 50 % eksperimentalnih osebkov. V naši raziskavi smo želeli ugotoviti, kolikšne so vrednosti LT50 pri nekaterih najpogostejših srednjeevropskih trogloksenih in troglofilnih vrstah med zimsko in poletno diapavzo, ločeno po spolih. V poskusu smo uporabili precizno termostatsko hladilno komoro THK/V1−2020 (Elestro, Slovenija) z nastavljivo temperaturo. Meritve smo začeli pri temperaturi −2,0 °C ter jih nadaljevali do temperature −12,0 °C, ko so poginili tudi na mraz najbolj odporni organizmi. Osebke smo izpostavila določeni temperaturi 24 ur, nakar so bili premeščeni za 48 ur v hladilnik s temperaturo 2,0 °C. Preživele osebke smo nato izpostavili za 1,0 °C nižji temperaturi in ponavljali postopek do temperature, pri kateri je kumulativno poginilo 50 % testnih osebkov določene vrste oziroma spola. Skladno s pričakovanji smo ugotovili, da imajo troglofilni organizmi, ki so bolje prilagojeni na diapavzo v hipogejičnih habitatih, višje vrednosti LT50 in obratno. Trogloksene so bolje prilagojene nestabilnemu epigejičnemu okolju in s tem občasno nižjim temperaturam, ter so bolj odporne na mraz. Vrednosti LT50 posameznih vrst se razlikujejo med različnimi razvojnimi stadiji, medtem ko so razlike med zimsko in poletno diapavzo zanemarljive. Odpornost na mraz ni pogojena s spolom. Cold–hardiness is the ability of organisms to survive shorter or longer exposure to lower temperatures than those causing their body fluids to freeze. This resistance to low temperatures can be measured by determining the LT50, which is the measure of temperature at 50% mortality of individuals exposed to below 0°C temperatures. In our investigation, our goal was to determine the LT50 values of selected, most widely spread Central European trogloxene and troglophile species during their winter and summer diapauses, separately by the sex. The experiment was carried out within a precise thermostatic cooling chamber THK/V1–2020 (Elestro, Slovenia) with adjustable temperature. We started the measurement at –2.0°C, and stopped it at –12.0°C, when all organisms died. The specimens were exposed to a particular temperature for a period of 24 hrs and afterwards they were hold for 48 hrs in a refrigerator at 2.0°C. The survived specimens were exposed to a 1.0°C lower temperature, and the procedure was consequently repeated until the LT50 was reached. As expected, we found out that troglophiles, which are better adapted to carry out a diapause in hypogean habitats, showed higher LT50 values and vice versa. Trogloxenes are better adapted to unstable epigean environment and to occasionally lower temperatures, and are consequently more cold hardy. The LT50 values of particular species differ between different developmental stages, while the difference between winter and summer diapauses is negligible. Resistance to cold is not sex–dependant. odpornost na mraz LT50 troglokseni troglofili diapavza cold–hardiness LT50 trogloxenes troglophiles diapause true true false [E. Antolinc] Slovenski jezik Angleški jezik Diplomsko delo 2009-02-24 08:40:12 2009-04-22 11:39:53 2022-04-11 15:48:01 2022-04-15 03:25:06 2009 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 57/59(043.2) 16749832 URN:SI:UM:DK:NCJP76UW UNI_Antolinc_Estera_2009.pdf UNI_Antolinc_Estera_2009.pdf 1 AD728FA6A25272CAF74A782C16E0CF9F 4aeb222cabcf90bbdcfaf7339cc91c86e5ad461ad64896d6e94f1d965202172e a3c997bb-7c02-11eb-bb7a-00155d0001ca 20.500.12556/dkum/f6973fdd-715c-432e-8802-a1cbcea37600 https://dk.um.si/Dokument.php?lang=slv&id=7503 Fakulteta za naravoslovje in matematiko 0 0 0