20.500.12556/DKUM-98712 Vpliv razvojne in kratkotrajne temperaturne aklimacije na toplotno toleranco podlubnika Ips typographus (Linnaeus, 1758) (Coleoptera: Curculionidae: Scolytinae) magistrsko delo Effect of developmental and short-term temperature acclimation on thermal tolerance in bark beetle ips typographus (linnaeus, 1758) (coleoptera: curculionidae: scolytinae) Populacijska dinamika osmerozobega smrekovega lubadarja (Ips typographus (Linnaeus, 1758)) je izrazito temperaturno odvisna, zato je razumevanje njegove fiziološke tolerance na temperaturne ekstreme ključno v kontekstu podnebnih sprememb. Namen raziskave je bil proučiti vpliv razvojne temperature ter kratkotrajne aklimacije odraslih osebkov na toplotno toleranco, izraženo kot toleranca na mraz in vročino. Osebke smo razvijali v smrekovih hlodih pri treh konstantnih temperaturah (17 °C, 24 °C in 31 °C), nato pa odrasle hrošče aklimirali pri enakih treh temperaturah, s čimer smo oblikovali devet kombinacij razvojnih in aklimacijskih režimov. Toleranco na mraz smo ovrednotili s časom okrevanja po ohladitveni komi (2 °C, 2 h), toleranco na vročino pa s časom do vročinske umiritve pri akutnem toplotnem stresu (43 °C). Rezultati so pokazali statistično značilen vpliv obeh dejavnikov na toplotno toleranco, pri čemer je bila aklimacija odraslih izrazitejši dejavnik. Pri testu tolerance na vročino je bila zaznana tudi značilna interakcija med razvojno in aklimacijsko temperaturo, saj je bila najvišja toleranca ugotovljena pri kombinaciji višjih temperatur. Ugotovitve potrjujejo visoko fenotipsko plastičnost odraslih osebkov ter poudarjajo pomen kratkotrajne aklimacije za preživetje v razmerah pogostejših temperaturnih ekstremov. The population dynamics of the eight-toothed spruce bark beetle (Ips typographus (Linnaeus, 1758)) are strongly temperature-dependent; therefore, understanding its physiological tolerance to temperature extremes is crucial in the context of climate change. The aim of this study was to investigate the effects of developmental temperature and short-term acclimation of adult individuals on thermal tolerance, expressed as cold and heat tolerance. Individuals were reared in spruce logs at three constant temperatures (17 °C, 24 °C and 31 °C), after which adult beetles were acclimated at the same three temperatures, resulting in nine combinations of developmental and acclimation regimes. Cold tolerance was assessed using recovery time from chill coma (2 °C for 2 h), while heat tolerance was evaluated by measuring the time to heat-induced immobilisation under acute thermal stress (43 °C). The results showed a statistically significant effect of both factors on thermal tolerance, with adult acclimation having a stronger influence. In the heat tolerance test, a significant interaction between developmental and acclimation temperature was also detected, with the highest tolerance observed in the combinations involving higher temperatures. These findings confirm the high phenotypic plasticity of adult individuals and highlight the importance of short-term acclimation for survival under conditions of increasingly frequent temperature extremes. Ips typographus razvojna temperatura aklimacija toleranca na mraz toleranca na vročino Ips typographus developmental temperature acclimation cold tolerance heat tolerance true true false [K. Skrivarnik] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-07-02 12:36:57 2026-07-17 11:57:39 2026-07-18 03:13:02 0000-00-00 00:00:00 2026 0 Maribor 0 Maribor X, 48 f. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 595.768.2:551.58(043.2) 285244931 EMAG_Skrivarnik_Kaja_2026.pdf EMAG_Skrivarnik_Kaja_2026.pdf 1 23DE5B957591CE40D004BBE4F44CCD6D f30e093fe4a71689106db5b2aae6c4fe12c06a6cb4887d42973b8758f7a9fe27 d03d1488-7605-11f1-a051-00155d580e34 https://dk.um.si/Dokument.php?lang=slv&id=207825 Fakulteta za naravoslovje in matematiko 0 0 0