20.500.12556/DKUM-99142 Functional genomics screening in Chlamydomonas reinhardtii maps the genetic landscape of tolerance to paraquat and diuron Functional genomics offers a powerful, unbiased approach to elucidating the molecular mechanisms underlying the toxicity of environmental pollutants. In this study, we applied genome-wide screening in Chlamydomonas reinhardtii to investigate two classical herbicides: paraquat and diuron. Our screen successfully uncovered critical nuclear-encoded pathways that govern susceptibility. For both herbicides, we identified genes regulating the assembly and maintenance of the photosynthetic machinery, highlighting the central role of nuclear control over these chloroplast-localized targets. Beyond these target-related factors, we discovered novel nontarget-site resistance mechanisms. For paraquat, we identified intracellular trafficking as the central determinant of toxicity, experimentally characterizing a P5B ATPase transporter and a fatty acid elongation pathway whose disruption, we propose, converges on the same endomembrane delivery route through sphingolipid depletion. In contrast, our screening data suggest that diuron tolerance may be associated with a metabolic strategy focused on energy conservation, where the inactivation of specific NADPH-consuming enzymes could preserve reducing power for essential antioxidant defense. Collectively, these findings demonstrate that functional genomics can reveal novel, complex modes of action even for well-characterized chemicals, providing the mechanistic resolution required to advance modern ecotoxicological risk assessments. parakvat diuron paraquat Genomika Herbicidi Ekotoksikologija true false true American Chemical Society Angleški jezik Slovenski jezik Delo ni kategorizirano 2026-08-04 07:17:00 2026-08-14 12:40:10 2026-08-15 03:05:44 0000-00-00 00:00:00 2026 0 0 str. 15570-15585 št. 22 Letn. 60 Jun. 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 2026-05-27 575.111 280635139 10.1021/acs.est.5c17308 1520-5851 Functional_genomics_screening_in_Ch-Godec-2026.pdf Functional_genomics_screening_in_Ch-Godec-2026.pdf 1 5AB9AECCE20BC82B3AB1F40E5EB0BC7A 8b7d462a34d352eca9a6d87a53c010d0b7bb6848d92f933551da6af609bdf75b 5794cd6b-97cc-11f1-910e-00155d0001be https://dk.um.si/Dokument.php?lang=slv&id=209509 https://pubs.acs.org/doi/10.1021/acs.est.5c17308 1 b94eed90-8fc3-11f1-8ff8-00155d0001be https://dk.um.si/Dokument.php?lang=slv&id=208889 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0