20.500.12556/DKUM-99579 Cytotoxicity assessment using label-free impedimetric biosensing comparative evaluation with conventional assays To address the global need for quantitative and sensitive methods to monitor the effects of chemical stress, we introduce a rapid, non-destructive impedimetric biosensor that uses in vitro cell-based systems as the biorecognition element and diazinon, an organophosphorus pesticide, as the model chemical for assessing stress. The study aimed to evaluate the biosensor's sensitivity and effectiveness relative to established in vitro cytotoxicity assays. Light microscopy confirmed cell density and viability on the electrodes, while electrochemical impedance spectroscopy (EIS) revealed a linear, concentration-dependent decrease in impedance following diazinon exposure. Crucially, the EIS biosensor demonstrated significantly lower effective concentrations (EC50 = 0.079 mM, EC20 = 0.012 mM, EC10 = 0.006 mM) than in conventional assays, which proves that our biosensor detects an earlier toxicological event. We conclude that the cell-based impedimetric biosensor provides a label-free platform for non-destructive assessment of the effects of aquatic contaminants. Electrochemical impedance spectroscopy (EIS) impedimetric biosensor MG-63 human osteosarcoma cell line chemical stressors diazinon kemija materiali biotehnologija elektrokemijska impedančna spektroskopija impedančni biosenzor MG-63 človeška celična linija osteosarkoma kemični stresorji diazinon true false true Elsevier Angleški jezik Slovenski jezik https://www.sciencedirect.com/journal/environmental-toxicology-and-pharmacology/vol/126/suppl/C Članek v reviji 2026-08-20 11:42:59 2026-08-20 11:42:59 2026-08-21 03:08:12 0000-00-00 00:00:00 2026 0 0 10 str. let. 126, št. članka 105128 15. avg. 2026 0000-00-00 Zaloznikova Objavljeno NiDoloceno 2026-05-21 2026-08-06 2026-08-15 544.6:602.1:576.3:615.9 288275971 10.1016/j.etap.2026.105128 1872-7077 1-s2.0-S1382668926002061-main.pdf 1-s2.0-S1382668926002061-main.pdf 1 C833859672E27D927807B9E3E5481D40 859c30d7aecdb6af366da78d462ae98318a1b4abfa479a0d394fe0405ef48067 f693208f-9c7b-11f1-b47c-00155d0001be https://dk.um.si/Dokument.php?lang=slv&id=209999 https://www.sciencedirect.com/science/article/pii/S1382668926002061 1 88cf1be6-9c7b-11f1-b47c-00155d0001be https://dk.um.si/Dokument.php?lang=slv&id=209998 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0