20.500.12556/DKUM-99600 Sinergistično delovanje selena in kadmija na rast ozkolistnega koščca Berula erecta Huds. v tkivni kulturi magistrsko delo Synergistic effect of selenium and cadmium on the growth of lesser water-parsnip Berula erecta Huds. in tissue culture Ozkolistni koščec (Berula erecta) je makrofit iz družine kobulnic s sposobnostjo hitre rasti in razvoja ter privzema različnih elementov in je zato zanimiv za fiziološke raziskave rastlin. Nizke koncentracije selena (Se) imajo dokazano pozitivne učinke tudi na rastline, zato smo raziskali ali lahko Se zaščiti B. erecta pred kadmijevim (Cd) stresom. B. erecta smo gojili na MS gojišču in jih po 14 dneh zalili z raztopinami z različnimi koncentracijami in kombinacijami Se (0,1 ali 1 mg L-1) in Cd (0 ali 10 ali 50 mg L-1). Močan negativen vpliv Cd v primerjavi s kontrolo brez Cd smo opazili pri najvišji koncentraciji 50 mg L-1 po štirih tednih kulture. Tudi 10 mg L-1 Cd je povzročalo rastlinam blažji stres. To se je kazalo v zmanjšani sveži masi, višini, dolžini korenin, količini klorofilov in antocianov. Najbolj spodbujevalen učinek Se na vse parametre je imel dodatek višje 1 mg L-1 koncentracija Se v MS gojišče brez Cd po štirih tednih kulture. Tam, kjer pa sta bila dodana 10 mg L-1 ali 50 mg L-1 Cd v gojišče, se je izrazil spodbujevalni učinek nižje 0,1 mg L-1koncentracija Se po štirih tednih kulture. S tem smo dokazali, da Se do neke mere deluje spodbujevalno ob stresnih dejavnikih, ki jih povzroča Cd stres. Obenem pa smo dokazali tudi, da višje koncentracije Se nimajo nujno pozitivnega učinka na rastlino, ampak se le ta lahko z elementom zasiči. Lesser water-parsnip (Berula erecta) is a macrophyte belonging to the Apiaceae family, characterised by rapid growth and development and by the uptake of various elements, and is therefore of interest for plant physiological research. Low concentrations of selenium (Se) have been shown to have positive effects on plants; we therefore investigated whether Se can protect B. erecta from cadmium (Cd) stress. We cultivated B. erecta on MS medium and, after 14 days, treated the plants with solutions containing various concentrations and combinations of Se (0.1 or 1 mg L⁻¹) and Cd (0, 10 or 50 mg L⁻¹). A strong negative effect of Cd, compared with the Cd-free control, was observed at the highest concentration of 50 mg L⁻¹ after four weeks of cultivation. Even 10 mg L⁻¹ Cd caused milder stress to the plants. This was reflected in reduced fresh weight, plant height, root length, and chlorophyll and anthocyanin contents. The most stimulatory effect of Se on all parameters was observed when a higher concentration of 1 mg L⁻¹ Se was added to the MS medium without Cd after four weeks of cultivation. However, when 10 mg L⁻¹ or 50 mg L⁻¹ Cd was added to the medium, the stimulatory effect was observed at the lower concentration of 0.1 mg L⁻¹ Se after four weeks of cultivation. We have thus demonstrated that selenium exerts a stimulatory effect, to a certain extent, in response to stress factors caused by cadmium. At the same time, we have also shown that higher concentrations of selenium do not necessarily have a positive effect on the plant, as the plant may simply become saturated with the element Apiaceae kadmij selenit Berula erecta toksičnost Apiaceae cadmium selenite Berula erecta toxicity true true false [L. Medik] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-08-20 20:45:47 2026-09-23 10:09:08 2026-09-24 03:07:30 0000-00-00 00:00:00 2026 0 Maribor 0 Maribor X, 35 str. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 581.5:582.795(043.2) 292120067 MAG_Medik_Lea_2026.pdf MAG_Medik_Lea_2026.pdf 1 39EBE85CFB98D233F802AB920E530765 d7ca30733dec3d6332305a8db1d0acc5a3a1c3d299fba904d8b6fba0e3dff9f8 3ae39b38-9cc9-11f1-b47c-00155d0001be https://dk.um.si/Dokument.php?lang=slv&id=210070 Fakulteta za naravoslovje in matematiko 0 0 0