20.500.12556/DKUM-99792 Biokemijska označitev izolatov pseudomonas spp. iz lezij listov murve (morus sp.) Biochemical characterization of pseudomonas spp. isolates from lesions of mulberry (morus sp.) leaves Bakterije rodu Pseudomonas vključujejo številne fitopatogene vrste, ki povzročajo gospodarsko pomembne bolezni rastlin. V diplomskem delu je predstavljena biokemijska označitev bakterijskih izolatov rodu Pseudomonas, pridobljenih iz lezij listov murve (Morus sp.). Eksperimentalni del je obsegal klasične biokemijske teste za ugotavljanje aktivnosti encimov želatinaze in arginin dihidrolaze ter sposobnosti razgradnje ogljikovih hidratov (glukoze, ksiloze, adonitola in manitola) ter tvorbo levana. Sintezo levana smo dodatno potrdili z analizo produktov kislinske hidrolize s tankoplastno kromatografijo (TLC). Rezultati so pokazali, da noben izmed testiranih izolatov Pseudomonas spp. ne razgrajuje želatine in ne sintetizira encima arginin dihidrolaze. Vsi izolati so bili sposobni presnavljati glukozo v aerobnih in anaerobnih razmerah, medtem ko adonitola ni razgrajeval nobeden izmed testiranih izolatov. Pri razgradnji manitola in ksiloze smo opazili raznolikost v biokemijskih lastnostih posameznih izolatov. Slovenski izolat Pseudomonas amygdali pv. mori, izolat MB01, teh dveh sladkorjev ni bil sposoben presnavljati, medtem ko so ga preostali trije izolati (Pseudomonas caspiana izolat KP01, Pseudomonas caspiana izolat KK01, Pseudomonas amygdali pv. mori izolat Ma001) lahko. S kromatografsko analizo TLC smo pri vseh izolatih uspešno potrdili sintezo zunajceličnega polisaharida levana, saj so se lise hidroliziranih vzorcev ujemale z referenčno liso fruktoze. Rezultati nakazujejo možno prilagajanje lokalnih populacij bakterij specifičnim mikrookoljskim razmeram ter potrjujejo, da biokemijska označitev sama po sebi ni zadostna za zanesljivo razlikovanje fitopatogenih psevdomonad, zato jo je smiselno dopolniti z analizo genetskih označevalcev. Bacteria belonging to the genus Pseudomonas include numerous phytopathogenic species that cause economically important plant diseases. This thesis presents the biochemical characterization of Pseudomonas spp. isolates obtained from lesions on mulberry (Morus sp.) leaves. The experimental work included classical biochemical tests to determine gelatinase and arginine dihydrolase activity, the ability to utilize carbohydrates (glucose, xylose, adonitol, and mannitol), and levan synthesis. Levan synthesis was further confirmed by analyzing the products of acid hydrolysis using thin-layer chromatography (TLC). The results showed that none of the tested isolates hydrolyzed gelatin or exhibited arginine dihydrolase activity. All isolates were capable of utilizing glucose under both aerobic and anaerobic conditions, whereas none of them utilized adonitol. Diversity in the biochemical characteristics of the isolates was observed in the utilization of mannitol and xylose. The Slovenian isolate Pseudomonas amygdali pv. mori MB01 was unable to utilize either of these carbohydrates, while the remaining three isolates (Pseudomonas caspiana KP01, Pseudomonas caspiana KK01, and Pseudomonas amygdali pv. mori Ma001) were able. TLC analysis confirmed the synthesis of the extracellular polysaccharide levan in all isolates, as the spots obtained from hydrolyzed samples corresponded to the reference fructose spot. The results suggest possible adaptation of local bacterial populations to specific microenvironmental conditions and confirm that biochemical characterization alone is insufficient for reliable differentiation of phytopathogenic pseudomonads. Therefore, it should be complemented by the analysis of genetic markers. Pseudomonas amygdali pv. mori Pseudomonas caspiana bakterijski ožig murve levan tankoplastna kromatografija (TLC) biokemijska označitev Pseudomonas amygdali pv. mori Pseudomonas caspiana mulberry bacterial blight levan thin-layer chromatography (TLC) biochemical characterization true true false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2026-08-26 13:49:55 2026-09-10 08:07:43 2026-09-11 03:08:02 0000-00-00 00:00:00 2026 0 Maribor 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 UN_Mrak_Alina_2026.pdf UN_Mrak_Alina_2026.pdf 1 4F75E9B8659757E1AB4D09FE148B6D19 65095d221a9724da4ed96a15d696ff4e78c1f08ac69e2917a6ba93f62590554e 1f879569-ac31-11f1-9c32-00155d0001be https://dk.um.si/Dokument.php?lang=slv&id=211946 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0