20.500.12556/DKUM-99892 Anatomske in ekofiziološke lastnosti prezimnih listov škrlatnordeče mrtve koprive (Lamium purpureum L.) v stresnih habitatih Anatomical and ecophysiological characteristics of overwintering leaves of purple dead-nettle (Lamium purpureum L.) in stressful habitats Za zagotovitev preživetja lahko nekatere rastline med drugim ohranijo funkcionalne liste skozi celotno zimsko obdobje. To so prezimni listi, ki so izpostavljeni številnim abiotskim dejavnikom, vključno z nizkimi temperaturami, visoko intenziteto svetlobe pozimi in zmanjšano dostopnostjo vode. Kljub temu vzdržujejo funkcionalnost fotosinteznega aparata, s čimer so povezane fiziološke in strukturne lastnosti listov. V okviru raziskave smo omenjene lastnosti proučili pri prezimnih listih škrlatnordeče mrtve koprive in jih primerjali med rastiščema ter mesecema. Fiziološko stanje smo ovrednotili z meritvami fluorescence klorofila a in vrednostmi SPAD, anatomske značilnosti pa z analizo prečnih rezov shranjenih listov in epidermalnih odtisov svežih listov (le pri rastlinah ob njivi v decembru). Preverili smo še povezanost izbranih fluorescenčnih parametrov z vrednostmi SPAD in izbranimi okoljskimi dejavniki. Podatki so bili statistično obdelani v programu RStudio. Med rastlinami ob njivi in ob cesti v marcu pri fizioloških parametrih nismo ugotovili statistično značilnih razlik, le eno na anatomski ravni, pri širini celic povrhnjice. Pri primerjavi rastlin ob njivi med marcem in decembrom so bile razlike izrazitejše predvsem na fiziološki ravni, medtem ko na anatomski niso ohranile statistične značilnosti. Vrednosti SPAD so bile statistično značilno povezane le s temotnim Fv/Fm v dveh skupinah, pri čemer je bil delež pojasnjene variabilnosti majhen. Izbrani fluorescenčni parametri niso bili statistično značilno povezani s svetlobnimi razmerami v nobeni od treh obravnavanih skupin, pri rastlinah ob njivi v decembru pa tudi ne s temperaturo. V slednji skupini sta bili svetloba in temperatura pozitivno povezani. S pomočjo anatomskih analiz smo potrdili bifacialno zgradbo listov in prisotnost nežleznih ter žleznih (peltatnih, kapitatnih) trihomov. Ugotovili smo, da so listne reže prisotne na obeh listnih površinah (amfistomatni listi). Epidermalni odtisi so pokazali izrazite razlike med zgornjo in spodnjo listno površino ter nasproten vzorec med gostoto in velikostjo listnih rež. Rezultati kažejo, da imajo prezimni listi škrlatnordeče mrtve koprive razmeroma podobno anatomsko zgradbo med preučevanima rastiščema in mesecema. Fiziološko stanje se je medtem izraziteje razlikovalo med marcem in decembrom, ne pa tudi med rastiščema. Ugotovljenih fluorescenčnih odzivov na podlagi rezultatov ni bilo mogoče ustrezno pojasniti z vrednostmi SPAD in okoljskimi dejavniki. To ensure survival, some plants can retain functional leaves throughout the winter. These overwintering leaves are exposed to various abiotic factors, including low temperatures, high winter light intensity, and reduced water availability. Despite these conditions, they maintain the functionality of the photosynthetic apparatus, and this process is associated with the physiological and structural characteristics of leaves. In this study, these characteristics were investigated in overwintering leaves of Lamium purpureum and compared across sampling sites and months. Physiological state was assessed using chlorophyll a fluorescence measurements and SPAD values. Anatomical characteristics were examined using cross-sections of preserved leaves and epidermal impressions of fresh leaves. Impressions were obtained only from leaves of plants growing beside the field in December. The relationships between selected fluorescence parameters, SPAD values, and selected environmental factors were also examined. Statistical analyses were performed using RStudio. No statistically significant differences in physiological parameters were found between plants growing beside the field and the road in March, while only one anatomical difference was detected in epidermal cell width. When plants growing beside the field were compared between March and December, differences were more pronounced at the physiological level, whereas anatomical differences did not remain statistically significant. SPAD values were significantly associated only with dark-adapted Fv/Fm in two groups, although the proportion of explained variance was small. The selected fluorescence parameters were not significantly associated with light conditions in any of the three groups, nor with temperature in plants growing beside the field in December. In this group, a positive association between light and temperature was observed. Anatomical analyses confirmed a bifacial leaf structure, amphistomatous leaves, and the presence of non-glandular, peltate glandular, and capitate glandular trichomes. Epidermal impressions showed pronounced differences between the adaxial and abaxial leaf surfaces and an inverse pattern between stomatal density and stomatal size. The results indicate that the overwintering leaves of Lamium purpureum have a relatively similar anatomical structure across studied sites and months. In contrast, physiological state differed more markedly between March and December, but not between sites. Based on the obtained results, it was not possible to adequately explain the observed fluorescence responses by SPAD values or the examined environmental factors. Lamium purpureum prezimni listi fiziološke lastnosti fluorescenca klorofila a SPAD anatomske lastnosti listne reže trihomi epidermalni odtis Lamium purpureum overwintering leaves physiological characteristics chlorophyll a fluorescence SPAD anatomical characteristics stomata trichomes epidermal impression true true false [M. Frešer] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2026-08-28 15:59:24 2026-09-28 09:45:13 2026-09-28 09:45:17 0000-00-00 00:00:00 2026 0 Maribor 0 IX, 73 str. 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 582.929.4:581.4:581.5(043.2) 292648195 MAG_Freser_Marisa_2026.pdf MAG_Freser_Marisa_2026.pdf 1 997D4A57463119CC5CA8175E1D9C93C2 182978a97e9a5dce2063b6137f81e7a1effd4936cb8437ec9417cd61d22c9017 58c7215c-a2eb-11f1-8903-00155d0001be https://dk.um.si/Dokument.php?lang=slv&id=210870 Fakulteta za naravoslovje in matematiko 0 0 0