| Naslov: | Determination of candesartan in human plasma with liquid chromatography - tandem mass spectrometry |
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| Avtorji: | ID Forjan, Vanja (Avtor)
ID Cvitkovič-Maričič, Lea (Avtor)
ID Prosen, Helena (Avtor)
ID Brodnjak-Vončina, Darinka (Avtor) |
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| Datoteke: |  Acta_Chimica_Slovenica_2016_Forjan_et_al._Determination_of_Candesartan_in_Human_Plasma_with_Liquid_Chromatography_–_Tandem_Mass_Spectrom.pdf (215,54 KB)
MD5: F89C8825CD8F11ED00C83A48B440704B
PID: 20.500.12556/dkum/669046f0-c2ff-4629-afeb-8268dc8968a0
 https://journals.matheo.si/index.php/ACSi/article/view/1836
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Znanstveno delo |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | FKKT - Fakulteta za kemijo in kemijsko tehnologijo
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| Opis: | A sensitive, specific and rapid liquid chromatography - tandem mass spectrometry method was developed and validated for the determination of candesartan in human plasma. Analyte was separated from endogenous components present in plasma by solid phase extraction. Chromatographic separation was performed on Gemini C18 analytical column using mobile phase acetonitrile – 5 mM ammonium formate pH 2 (90:10, v/v) at flow rate of 0.3 mL/min. For detection, tandem mass spectrometry in SRM mode with positive electrospray ionization was used. The mass transitions m/z 441.1 > 263.1 and 445.1 > 267.1 were used to determine candesartan by using candesartan-d4 as an internal standard. After development, the method was validated according to the requirements of EMA regulatory guidelines in the concentration range 1 - 400 ng/ml in human plasma. Limit of quantification (LLOQ) was 1 ng/ml. The developed and validated method proved to be very fast and reproducible and was therefore successfully implemented in pharmacokinetic and bioequivalence studies with large number of study samples. |
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| Ključne besede: | candesartan, liquid chromatography, tandem mass spectrometry, human plasma |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Leto izida: | 2016 |
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| Št. strani: | str. 38-46 |
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| Številčenje: | Letn. 63, št. 1 |
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| PID: | 20.500.12556/DKUM-67425  |
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| ISSN: | 1318-0207 |
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| UDK: | 543.544.5 |
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| COBISS.SI-ID: | 1536821699 |
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| DOI: | 10.17344/acsi.2015.1836 |
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| ISSN pri članku: | 1318-0207 |
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| NUK URN: | URN:SI:UM:DK:QC9RBESG |
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| Datum objave v DKUM: | 17.08.2017 |
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| Število ogledov: | 1694 |
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| Število prenosov: | 413 |
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| Metapodatki: |    |
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| Področja: | Ostalo
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