| Naslov: | Synthesis of betaine, choline and carnitine containing polymers for dermal wound healing |
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| Avtorji: | ID Jurko, Lucija (Avtor)
ID Kargl, Rupert (Mentor) Več o mentorju...  |
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| Datoteke: |  DOK_Jurko_Lucija_2024.pdf (16,36 MB)
MD5: 9BD7E5B87A801B4D18367B9492F5B4AF
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| Jezik: | Slovenski jezik |
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| Vrsta gradiva: | Doktorsko delo/naloga |
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| Tipologija: | 2.08 - Doktorska disertacija |
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| Organizacija: | FS - Fakulteta za strojništvo
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| Opis: | In this study, we explored the development of cationized 2-hydroxyethyl cellulose (HEC) and succinylated polyallylamine (PAA) in conjunction with polyvinyl alcohol (PVA) for potential applications as antimicrobial wound dressings. Quaternary ammonium compounds are known for their broad-spectrum antimicrobial properties against both Gram-negative and Gram-positive bacteria, but their non-selectivity can lead to high cytotoxicity. To improve the biocompatibility of the cationic materials investigates two distinct approaches. The first approach involves reducing the cationic nature of protonated PAA at a pH below 8.3 through amidation reaction with succinic anhydride. Gradual reduction of the cationic charge is achieved by varying the molar ratios of succinic anhydride during the reaction process. Notably, this reaction can be carried out in an aqueous solution, eliminating potential issues associated with solvent removal. The second part of this thesis focuses on introducing cationic charge by covalently binding naturally occurring quaternary ammonium compounds, such as betaine hydrochloride (BET HCl), choline chloride (ChCl), and carnitine hydrochloride (carnitine). Using 1,1′-carbonyldiimidazole (CDI), we quaternized HEC in anhydrous DMSO, with a detailed investigation of the reaction mechanism by isolating and characterizing intermediate products. This same procedure was applied to form a cationic dimer between BET HCl and ChCl. The chemical structures of the resulting materials were characterized using attenuated total reflectance infrared spectroscopy (ATR-IR) and nuclear magnetic resonance (NMR). The quantification of cationic and total charge was determined through polyelectrolyte and potentiometric titration, respectively. While succinylated PAA exhibited the anticipated biological properties associated with the reduction of cationic characteristics, the same behaviour was not observed for quaternized materials. High cytotoxicity and low antimicrobial properties in the derivatized HEC may be attributed to inadequate purification and the sensitivity of fibroblasts. We produced electrospun non-woven mats incorporating PVA and these derived materials using a single-needle electrospinning machine. The average diameter of the fibres was determined through scanning electron microscopy (SEM) images. Despite the cytotoxicity and the absence of significant antimicrobial properties in these materials, they exhibit potential as effective cationic flocculants for wastewater treatment. Further investigations are pending to address purification issues and make slight adjustments to the procedure to potentially scale up production. These materials still hold promise for applications in both biomedical and environmental chemistry. |
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| Ključne besede: | cellulose, quaternary ammonium compounds, antimicrobial, cytotoxicity, electrospinning |
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| Kraj izida: | Maribor |
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| Založnik: | [L. Jurko] |
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| Leto izida: | 2024 |
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| PID: | 20.500.12556/DKUM-86596 |
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| UDK: | [604.4:615.33]:547.458.8(043.3) |
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| COBISS.SI-ID: | 203016195 |
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| Datum objave v DKUM: | 11.07.2024 |
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| Število ogledov: | 345 |
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| Število prenosov: | 79 |
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| Metapodatki: |    |
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| Področja: | KTFMB - FS
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