| Naslov: | Autolysis affects the iron cargo of ferritins in neurons and glial cells at different rates in the human brain |
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| Avtorji: | ID Sunkara, Sowmya (Avtor)
ID Radulović, Snježana (Avtor)
ID Lipovšek Delakorda, Saška (Avtor)
ID Birkl, Christoph (Avtor)
ID Eggenreich, Stefan (Avtor)
ID Birkl-Toeglhofer, Anna Maria (Avtor)
ID Schinagl, Maximilian (Avtor)
ID Funk, Daniel (Avtor)
ID Stöger-Pollach, Michael (Avtor)
ID Haybaeck, Johannes (Avtor)
ID Gössler, Walter (Avtor)
ID Ropele, Stefan (Avtor)
ID Leitinger, Gerd (Avtor) |
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| Datoteke: |  Sunkara-2023-Autolysis_Affects_the_Iron_Cargo.pdf (2,73 MB)
MD5: 49CF3BD66E8C03AA2EDE8A7E428D93A3
 https://doi.org/10.1007/s10571-023-01332-w
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Članek v reviji |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | FNM - Fakulteta za naravoslovje in matematiko
MF - Medicinska fakulteta
FKKT - Fakulteta za kemijo in kemijsko tehnologijo
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| Opis: | Iron is known to accumulate in neurological disorders, so a careful balance of the iron concentration is essential for healthy brain functioning. An imbalance in iron homeostasis could arise due to the dysfunction of proteins involved in iron homeostasis. Here, we focus on ferritin—the primary iron storage protein of the brain. In this study, we aimed to improve a method to measure ferritin-bound iron in the human post-mortem brain, and to discern its distribution in particular cell types and brain regions. Though it is known that glial cells and neurons differ in their ferritin concentration, the change in the number and distribution of iron-filled ferritin cores between different cell types during autolysis has not been revealed yet. Here, we show the cellular and region-wide distribution of ferritin in the human brain using state-of-the-art analytical electron microscopy. We validated the concentration of iron-filled ferritin cores to the absolute iron concentration measured by quantitative MRI and inductively coupled plasma mass spectrometry. We show that ferritins lose iron from their cores with the progression of autolysis whereas the overall iron concentrations were unaffected. Although the highest concentration of ferritin was found in glial cells, as the total ferritin concentration increased in a patient, ferritin accumulated more in neurons than in glial cells. Summed up, our findings point out the unique behaviour of neurons in storing iron during autolysis and explain the differences between the absolute iron concentrations and iron-filled ferritin in a cell-type-dependent manner in the human brain. |
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| Ključne besede: | ferritin, human brain, energy-filtered transmission electron microscopy, quantitative magnetic resonance imaging, autolysis |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Poslano v recenzijo: | 06.12.2022 |
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| Datum sprejetja članka: | 27.02.2023 |
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| Datum objave: | 15.03.2023 |
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| Založnik: | Springer Nature |
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| Leto izida: | 2023 |
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| Št. strani: | Str. 2909-2923 |
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| Številčenje: | Letn. 43, Št. 6 |
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| PID: | 20.500.12556/DKUM-87536  |
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| UDK: | 577 |
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| COBISS.SI-ID: | 145448963 |
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| DOI: | 10.1007/s10571-023-01332-w |
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| ISSN pri članku: | 0272-4340 |
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| Datum objave v DKUM: | 20.03.2024 |
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| Število ogledov: | 362 |
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| Število prenosov: | 53 |
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| Metapodatki: |    |
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| Področja: | Ostalo
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