| Naslov: | Ultrafast multicellular calcium imaging of calcium spikes in mouse beta cells in tissue slices |
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| Avtorji: | ID Dolenšek, Jurij (Avtor)
ID Pohorec, Viljem (Avtor)
ID Skelin, Maša (Avtor)
ID Gosak, Marko (Avtor)
ID Stožer, Andraž (Avtor) |
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| Datoteke: |  RAZ_Dolensek_Jurij_2025.pdf (9,70 MB)
MD5: DF3AE70739E4403A5B976D6665FE8394
 https://onlinelibrary.wiley.com/doi/10.1111/apha.14261
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Znanstveno delo |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | MF - Medicinska fakulteta
FNM - Fakulteta za naravoslovje in matematiko
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| Opis: | Background: The crucial steps in beta cell stimulus-secretion coupling upon stimulation with glucose are oscillatory changes in metabolism, membrane potential, intracellular calcium concentration, and exocytosis. The changes in membrane potential consist of bursts of spikes, with silent phases between them being dominated by membrane repolarization and absence of spikes. Assessing intra- and intercellular coupling at the multicellular level is possible with ever-increasing detail, but our current ability to simultaneously resolve spikes from many beta cells remains limited to double-impalement electrophysiological recordings. Methods: Since multicellular calcium imaging of spikes would enable a better understanding of coupling between changes in membrane potential and calcium concentration in beta cell collectives, we set out to design an appropriate methodological approach. Results: Combining the acute tissue slice method with ultrafast calcium imaging, we were able to resolve and quantify individual spikes within bursts at a temporal resolution of >150 Hz over prolonged periods, as well as describe their glucose-dependent properties. In addition, by simultaneous patch-clamp recordings we were able to show that calcium spikes closely follow membrane potential changes. Both bursts and spikes coordinate across islets in the form of intercellular waves, with bursts typically displaying global and spikes more local patterns. Conclusions: This method and the associated findings provide additional insight into the complex signaling within beta cell networks. Once extended to tissue from diabetic animals and human donors, this approach could help us better understand the mechanistic basis of diabetes and find new molecular targets. |
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| Ključne besede: | beta cell, calcium imaging, calcium oscillations, calcium spikes, physiology |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Poslano v recenzijo: | 29.11.2024 |
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| Datum sprejetja članka: | 16.01.2024 |
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| Datum objave: | 01.02.2025 |
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| Založnik: | John Wiley & Sons Ltd |
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| Leto izida: | 2025 |
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| Št. strani: | Str. 1-20 |
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| Številčenje: | Letn. 241, Št. 2 |
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| PID: | 20.500.12556/DKUM-91652  |
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| UDK: | 612 |
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| COBISS.SI-ID: | 222131203 |
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| DOI: | 10.1111/apha.14261 |
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| ISSN pri članku: | 1748-1716 |
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| Datum objave v DKUM: | 24.01.2025 |
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| Število ogledov: | 127 |
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| Število prenosov: | 16 |
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| Metapodatki: |    |
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| Področja: | Ostalo
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