| Naslov: | Comparison of conventional and supercritical isolation methods from bee pollen and their effects on the concentrations of bioactive compounds |
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| Avtorji: | ID Žitek Makoter, Taja (Avtor)
ID Postružnik, Vesna (Avtor)
ID Kyriakoudi, Anastasia (Avtor)
ID Pantazi, Elli (Avtor)
ID Kalogiouri, Natasa (Avtor)
ID Mourtzinos, Ioannis (Avtor)
ID Knez, Željko (Avtor)
ID Knez Marevci, Maša (Avtor) |
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| Datoteke: |  1-s2.0-S0896844625000865-main.pdf (6,76 MB)
MD5: AAB80705C77E0C8578527BB30EF7A2E3
 https://www.sciencedirect.com/science/article/pii/S0896844625000865?via%3Dihub
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Članek v reviji |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | FKKT - Fakulteta za kemijo in kemijsko tehnologijo
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| Opis: | The present work represents a profound investigation of material pretreatment, comparison of different isolation methods (conventional and supercritical), and characterization of phenolic compounds and amino acids from bee pollen. The determination of amino acids was carried out after developing a novel hydrophilic interaction chromatography combined with quadrupole time-of-flight tandem mass spectrometric method (HILIC-QTOF-MS/MS) with limits of detection over the range 1 mg/kg -5 mg/kg and recoveries over the range 87.6% - 97.1%. The optimal conditions for the isolation of amino acids are in the range of temperatures between 25°C to 55°C and at pressures between 300 bar and 350 bar. It can be concluded that the extraction temperature and the pressure affect antioxidant activity. The favored conditions are lower temperatures and higher pressures. |
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| Ključne besede: | bee pollen, in vitro antioxidant activity, HILIC-LC-QTOF-MS.MS, amino acids, WM-266-4 cells |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Poslano v recenzijo: | 18.10.2024 |
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| Datum sprejetja članka: | 14.03.2025 |
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| Datum objave: | 18.03.2025 |
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| Založnik: | Elsevier |
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| Leto izida: | 2025 |
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| Št. strani: | 34 str. |
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| PID: | 20.500.12556/DKUM-92192  |
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| UDK: | 66.02 |
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| COBISS.SI-ID: | 229553667 |
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| DOI: | 10.1016/j.supflu.2025.106599 |
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| ISSN pri članku: | 1872-8162 |
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| Avtorske pravice: | © 2025 The Author(s) |
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| Datum objave v DKUM: | 20.03.2025 |
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| Število ogledov: | 159 |
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| Število prenosov: | 10 |
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| Metapodatki: |    |
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| Področja: | Ostalo
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