| Naslov: | Bioactive bacterial nanocellulose membranes for non-surgical debridement and infection prevention in burn wound healing |
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| Avtorji: | ID Jančič, Urška (Avtor)
ID Nacu, Isabella (Avtor)
ID Vereştiuc, Liliana (Avtor)
ID Rancan, Fiorenza (Avtor)
ID Gorgieva, Selestina (Avtor) |
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| Datoteke: |  1-s2.0-S266689392500101X-main.pdf (24,82 MB)
MD5: C9584C9A6655E876A35E2F9520D70A69
 https://www.sciencedirect.com/science/article/pii/S266689392500101X
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Članek v reviji |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | FS - Fakulteta za strojništvo
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| Opis: | Novel bioactive bacterial nanocellulose (BnC) membranes were developed for effective, non-surgical debridement and infection-prevention in burn wound healing. Membranes were modified in situ with carboxymethyl cellulose (CMC) and ex situ with the proteolytic enzyme bromelain (Br) and antimicrobial peptide nisin (N). Post-processing into stable cellulose nanocrystal dispersions (ζ = -26 mV), enables assembly of model films for demonstration of high, irreversible bromelain (95 %) and nisin (99.5 %) adsorption. The BnC-CMC and BnC-CMC-N membranes were in vitro cytocompatible for HaCaT cells and induced faster cell proliferation with cell viability exceeding 100 % after 24 h incubation. The innovative aspect of this study lies in the ex vivo evaluation using an advanced human skin explant model with induced burns, providing a realistic, physiologically relevant assessment of membrane performance. Ex vivo experiments indicated the cytocompatibility of the BnC-CMC membrane with no acute toxicity or skin irritation, while nisin presence resulted in moderate irritating effect. Notably, the BnC-CMC-Br membrane showed digestion of intercellular junctions in the epidermis, while not inducing acute toxicity and skin irritation. By leveraging this innovative ex vivo human skin model in novel BnC-based membranes testing, the study provides a crucial translational step, bridging in vitro assessments and clinical applications for burn wound treatment. |
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| Ključne besede: | bacterial nanocellulose, Bromelain, Nisin, Carboxymethyl cellulose, antimicrobial function, bioactive, burn wound treatment |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 15.03.2025 |
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| Založnik: | Elsevier |
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| Leto izida: | 2025 |
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| Št. strani: | 20 str. |
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| Številčenje: | Vol. 10, [article no.] 100762 |
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| PID: | 20.500.12556/DKUM-92364  |
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| UDK: | 677.46:620.3 |
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| COBISS.SI-ID: | 231067907 |
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| DOI: | 10.1016/j.carpta.2025.100762 |
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| ISSN pri članku: | 2666-8939 |
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| Avtorske pravice: | © 2025 The Author(s) |
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| Datum objave v DKUM: | 01.04.2025 |
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| Število ogledov: | 196 |
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| Število prenosov: | 16 |
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| Metapodatki: |    |
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| Področja: | Ostalo
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