| Naslov: | The effect of combined elicitation with light and temperature on the chlorogenic acid content, total phenolic content and antioxidant activity of berula erecta in tissue culture |
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| Avtorji: | ID Senekovič, Jan (Avtor)
ID Ciringer, Terezija (Avtor)
ID Ambrožič-Dolinšek, Jana (Avtor)
ID Islamčević Razboršek, Maša (Avtor) |
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| Datoteke: |  plants-13-01463.pdf (1,81 MB)
MD5: 479F7DE4A9BEA16ADBD53AB08701D6D4
 https://www.mdpi.com/2223-7747/13/11/1463
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Članek v reviji |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | FKKT - Fakulteta za kemijo in kemijsko tehnologijo
FKBV - Fakulteta za kmetijstvo in biosistemske vede
FNM - Fakulteta za naravoslovje in matematiko
PEF - Pedagoška fakulteta
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| Opis: | Chlorogenic acid is one of the most prominent bioactive phenolic acids with great pharmacological, cosmetic and nutritional value. The potential of Berula erecta in tissue culture was investigated for the production of chlorogenic acid and its elicitation combined with light of different wavelengths and low temperature. The content of chlorogenic acid in the samples was determined by HPLC-UV, while the content of total phenolic compounds and the antioxidant activity of their ethanol extracts were evaluated spectrophotometrically. The highest fresh and dry biomasses were obtained in plants grown at 23 °C. This is the first study in which chlorogenic acid has been identified and quantified in Berula erecta. The highest chlorogenic acid content was 4.049 mg/g DW. It was determined in a culture grown for 28 days after the beginning of the experiment at 12 °C and under blue light. The latter also contained the highest content of total phenolic compounds, and its extracts showed the highest antioxidant activity. Berula erecta could, potentially, be suitable for the in vitro production of chlorogenic acid, although many other studies should be conducted before implementation on an industrial scale. |
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| Ključne besede: | Berula erecta, Apiaceae, plant tissue culture, elicitation, phenolic compounds, chlorogenic acid, antioxidant activity |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Poslano v recenzijo: | 14.03.2024 |
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| Datum sprejetja članka: | 23.05.2024 |
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| Datum objave: | 25.05.2024 |
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| Založnik: | MDPI |
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| Leto izida: | 2024 |
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| Št. strani: | 20 str. |
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| Številčenje: | Vol. 13, iss. 11, [article no.] 1463 |
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| PID: | 20.500.12556/DKUM-96776  |
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| UDK: | 66.02 |
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| COBISS.SI-ID: | 197046019 |
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| DOI: | 10.3390/plants13111463 |
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| ISSN pri članku: | 2223-7747 |
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| Avtorske pravice: | © 2024 by the authors |
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| Datum objave v DKUM: | 28.01.2026 |
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| Število ogledov: | 192 |
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| Število prenosov: | 3 |
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| Metapodatki: |    |
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| Področja: | Ostalo
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