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Title:Interakcije med piščančjimi intestinalnimi epitelnimi celicami B1OXI in bakterijami Campylobacter jejuni
Authors:ID Novak, Vesna (Author)
ID Cencič, Avrelija (Mentor) More about this mentor... New window
Files:.pdf UNI_Novak_Vesna_2009.pdf (1,51 MB)
MD5: AE0933C9CD5F63070B55463D3BA6A0F4
PID: 20.500.12556/dkum/0eb69d27-1e81-433d-9198-2df106b7348d
 
Language:Slovenian
Work type:Undergraduate thesis
Organization:FKBV - Faculty of Agriculture and Life Sciences
Abstract:Bakterije rodu Campylobacter jejuni uvrščamo med glavne povzročitelje črevesnih enteritisov. Najpogostejši način okužbe s kampilobakterji predstavlja zaužitje kontaminirane hrane, predvsem nezadostno toplotno obdelanega piščančjega mesa, svežega mleka in kontaminirane vode. V nalogi smo preučevali znotrajcelično preživelost, adhezivnost ter invazivnost bakterij v piščančjih intestinalnih celicah. Rezultati so pokazali, da imajo največjo adhezivnost in invazivnost pri piščančjih intestinalnih epitelijskih celicah B1OXI bakterije Campylobacter jejuni, in sicer po 10 h infekcije. Pri prašičjih PSI celicah, pa imajo bakterije ob kratkotrajni izpostavitvi atmosferske koncentracije kisika ali pa, ko niso izpostavljene stresnim pogojem največjo adhezivnost in invazivnost. Potencial je pri vseh okuženih adheriranih bakterijah negativen ali pa je blizu 0 V. Za invadirane celice velja, da je potencial največji pri razredčinah, po 10 h infekcije. Poskusi za prašičje celice kažejo, da Campylobacter jejuni povzroča majhne spremembe v TER, in sicer pri 10 urah po infekciji. Tam, kjer gentamicin ni uporabljen, pa lahko najdemo bakterije še 24 h po okužbi. Iz dobljenih rezultatov lahko rečemo, da piščanci za bakterijo Campylobacter jejuni ne zbolijo tako kot prašiči, ampak so le prenašalci te bolezni.
Keywords:Campylobacter jejuni, adhezija, invazija, rezistenca, potencial
Place of publishing:Maribor
Year of publishing:2009
PID:20.500.12556/DKUM-10896 New window
NUK URN:URN:SI:UM:DK:BOI4F1DJ
Publication date in DKUM:29.07.2009
Views:4318
Downloads:393
Metadata:XML DC-XML DC-RDF
Categories:FKBV
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Secondary language

Language:English
Title:Interaction between chicken intestinal epithelial cells B1OXI and the bacteria Campylobacter jejuni
Abstract:Bacteria Campylobacter jejuni is a major causes of intestinal enteritisov. The method of infection with C. jejuni represents the ingestion of contaminated food, especially poor heat-treated chicken meat, fresh milk and contaminated water. We have studied intracellular survive, adhesion and invasion of bacteria in chicken intestinal cells. The results show, that the highest adhesion and invasion in the chicken intestinal epithelial B1OXI cells of Campylobacter are after 10 hours of infection. In PSI pig cells, has C. jejuni the highetest adhesion and invasion at the short-term exposure to atmospheric concentrations of oxygen, or when they are not exposed to stressful conditions. The potential in adhension bacteria is negative, or is close to 0 volts. In invasion cells is the highest by the dilutions after 10 hours of infection Results in experiments with the pig cells show, that C. jejuni has a small changes in TER after 10 hours infection. When we wasn`t used gentamicin, there we can found the bacteria 24 hours after infection. We can say, that the chickens do not suffer with the bacteria C jejuni such as pigs, but they are only carriers of this disease.
Keywords:Campylobacter jejuni, adhesion, invasion, resistance, potential


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