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Title:
Izolacija in biološki učinki potencialnih protektivnih oziroma probiotičnih bakterij v kozjem mleku in intestinalnem traktu
Authors:
ID
Trapečar, Martin
(
Author
)
ID
Cencič, Avrelija
(
Mentor
)
More about this mentor...
Files:
UNI_Trapecar_Martin_2009.pdf
(1,49 MB)
MD5: C665DBC2B7A6B273F96BBF28ABD30BC0
PID:
20.500.12556/dkum/c67f32a8-6a32-40fb-b7fc-6b4d678a4a64
Language:
Slovenian
Work type:
Undergraduate thesis
Organization:
FKBV - Faculty of Agriculture and Life Sciences
Abstract:
Iz vzorcev kozjega mleka in fecesa smo uspešno izolirali 16 bakterijskih vzorcev in jih karakterizirali s pomočjo klasičnih in sodobnih mikrobioloških metod. Izmed izoliranih in karakteriziranih 16 bakterij jih 14 pripada rodu Lactobacillus ter 2 rodu Lactococcus. Dokazali smo pozitiven vpliv bakterij v in vitro celičnem modelu intestinalnega trakta. Rezultati so pokazali dobro stopnjo vezave na intestinalni epitelij, sorazmerno nizko citotoksičnost ter spodbujanje nespecifičnega imunskega odziva. S tem smo dokazali tudi zadostnost naših sevov za selekcijske kriterije. So funkcionalni, predvsem pa ne patogeni. Zanimivi za nadaljnje raziskave so predvsem vzorci M5K1 (Lactobacillus Brevis), F1K1K2 (Lactobacillus Plantarum) ter F1K1K1 (Lactococcus Rafinolactis), ki so najbolj izstopali po učinkovitosti. Navkljub dejstvu, da so vsi izolirani sevi pripadniki mlečnokislinskih bakterij je opazna vrstna specifičnost posameznih izoliranih sevov, ki se med seboj močno razlikujejo glede biološke aktivnosti. Prav tako prihaja do razhajanj znotraj posamezne vrste. Naše delo predstavlja v svetu edinstven eksperimentalni model za tovrstne analize.
Keywords:
koze / probiotiki / celični model / vezava / imunski odziv
Place of publishing:
Maribor
Year of publishing:
2009
PID:
20.500.12556/DKUM-12226
NUK URN:
URN:SI:UM:DK:BTGQKXVF
Publication date in DKUM:
20.10.2009
Views:
3216
Downloads:
263
Metadata:
Categories:
FKBV
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Secondary language
Language:
English
Title:
Isolation and biological activity of potentially protective or probiotic bacteria in goat milk and intestinal tract
Abstract:
We isolated and characterized successfully 16 bacterial samples from goat milk and feces with classical and modern practices. Among the isolated bacteria, 14 belong to the genus of Lactobacillus and 2 belong to the genus of Lactococcus. We proved the beneficial influence of bacteria in a in vitro intestinal cell model. The results showed a good degree of attachment to the intestinal epithelium, low citotoxicity and stimulation of non-specific immune responses. With this we also showed that these bacteria fulfill the criteria for probiotic selection. They are functional and non-pathogenic. The isolates M5K1 (Lactobacillus Brevis), F1K1K2 (Lactobacillus Plantarum) and F1K1K1 (Lactococcus Rafinolactis) are especially interesting for further research due to their efficiency. We observed strong specifities among different genus groups concerning their bio-activity. Our work presents a unique experimental approach for this kind of analysis.
Keywords:
goats / probiotics / cell model / attachment / immune response
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