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Title:IZOLACIJA IN KARAKTERIZACIJA BAKTERIJ IZ RIZOPLANA HLADNIKIJE
Authors:ID Mithans, Tamara (Author)
ID Rupnik, Maja (Mentor) More about this mentor... New window
ID Ambrožič Dolinšek, Jana (Comentor)
Files:.pdf UNI_Mithans_Tamara_2009.pdf (2,24 MB)
MD5: 094F11CB0B384BF68BC60BB235847A90
PID: 20.500.12556/dkum/d5be87e2-d3a1-4786-969e-87c7e6e1be2d
 
Language:Slovenian
Work type:Undergraduate thesis
Organization:FNM - Faculty of Natural Sciences and Mathematics
Abstract:Na površini korenin, imenovani rizoplan, lahko najdemo zelo različne skupine mikroorganizmov. Ti lahko na rast rastlin vplivajo pozitivno, lahko pa s svojo prisotnostjo in delovanjem rastlini škodujejo. Cilj diplomskega dela je bil izolacija in identifikacija bakterij, ki naseljujejo rizoplan hladnikije — slovenske endemične vrste. Iz treh rastlin smo osamili 17 bakterijskih sevov. Izolirali smo bakterijsko DNA in s pomočjo verižne reakcije s polimerazo (PCR) pomnožili 16S rDNA. Glede na primerjavo pomnožene 16S rDNa z referenčnimi bazami podatkov (GenBank, Ribosomal database project) smo izolirane bakterije identificirali. Izolirane bakterije so pripadale rodovom Bacillus, Enterobacter, Pseudomonas, Erwinia in Pantoea.
Keywords:Hladnikija, Hladnikia pastinacifolia, 16S rDNA, PCR, verižna reakcija s polimerazo, rizosfera, rizoplan, rizobakterije
Place of publishing:Maribor
Publisher:[T. Mithans]
Year of publishing:2009
PID:20.500.12556/DKUM-12234 New window
UDC:57/59(043.2)
COBISS.SI-ID:17242632 New window
NUK URN:URN:SI:UM:DK:DKOILT6K
Publication date in DKUM:25.11.2009
Views:4042
Downloads:378
Metadata:XML DC-XML DC-RDF
Categories:FNM
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Secondary language

Language:English
Title:Isolation and characterization of bacteria from Rhizoplane of Hladnikia pastinacifolia
Abstract:On the surface of roots called rhizoplane various groups of microorganisms are present. They can have plant growth promoting effects, but their presence and activity can also have inhibit the plant growth. The aim of the thesis was the isolation and identification of bacteria, which dwell in the rhizoplane of the Slovene endemic genus Hladnikia. From three plants we isolated17 bacterial strains. We isolated the DNA from the bacteria and amplified 16S rDNA regions with polymerase chain reaction (PCR); The identification of the strains was done by compariosn of 16S rDNa sequences with reference databases GenBank and Ribosomal Database Project. The isolated bacteria belonged to the genera Bacillus, Enterobacter, Pseudomonas, Erwinia and Pantoea.
Keywords:Hladnikia pastinacifolia, 16S rDNA, PCR, polymerase chain reaction, rhizospere, rhizoplane, rhizobacteria


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