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Title:Vpliv jasmonske kisline, ranitve in organsko revnega gojišča na regeneracijo listov praproti Platycerium bifurcatum (Cav.) C. Chr. v tkivni kulturi
Authors:ID Antolin, Ksenija (Author)
ID Ambrožič-Dolinšek, Jana (Mentor) More about this mentor... New window
Files:.pdf UNI_Antolin_Ksenija_2011.pdf (1,94 MB)
MD5: 44CE83EDF553088197CACC57C1239D9C
PID: 20.500.12556/dkum/e59e036c-da76-435d-9034-ee9f905c32fe
 
Language:Slovenian
Work type:Undergraduate thesis
Organization:FNM - Faculty of Natural Sciences and Mathematics
Abstract:Raziskali smo vpliv jasmonske kisline (JA) in ranitve ter sočasni učinek obeh dejavnikov na regeneracijo listov praproti Platycerium bifurcatum v tkivni kulturi. V MS gojišče z 0,01% saharoze ter z 0, 0.1, 1, 10 in 100 µM JA smo polagali neranjene ločene liste ter ločene liste, ki smo jih različno močno ranili. Po 50 dneh kulture smo na listih spremljali razvoj novih struktur: brstov, rizoidov, apospornih gametofitov in intermediatov. Ugotovili smo, da nižje koncentracije JA pospešujejo razvoj brstov, rizoidov ter intermediatov, medtem ko višje koncentracije vzpodbudijo razvoj apospornih gametofitov. Na listih z ranjeno zgornjo povrhnjico se je razvilo največje število sporofitskih struktur (brstov in rizoidov) ter intermediatov, medtem ko je bil razvoj apospornih gametofitov najbolj pospešen na nasekljanih listih. Regeneracija listne kulture pod vplivom različnih koncentracij eksogeno dodane JA je bila podobna regeneraciji, sproženi kot odziv na različne stopnje ranitve.
Keywords:Platycerium bifurcatum, jasmonska kislina, ranitev, brsti, rizoidi, aposporni gametofiti, intermediati
Place of publishing:Maribor
Publisher:[K. Antolin]
Year of publishing:2011
PID:20.500.12556/DKUM-18928 New window
UDC:57/59(043.2)
COBISS.SI-ID:18467592 New window
NUK URN:URN:SI:UM:DK:5QU8XNWN
Publication date in DKUM:22.06.2011
Views:3452
Downloads:125
Metadata:XML DC-XML DC-RDF
Categories:FNM
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Secondary language

Language:English
Title:The effect of jasmonic acid, wounding and organically deficient medium on regeneration of Platycerium bifurcatum (Cav.) C. Chr. fern leaves in tissue culture
Abstract:The effect of jasmonic acid (JA), wounding and simultaneous influence of both parameters on regeneration of Platycerium bifurcatum fern leaves in tissue culture were studied. Intact separate leaves and separate leaves that were wounded to varying degrees were placed flat on MS medium supplemented with 0.01% sucrose and with 0, 0.1, 1, 10 in 100 µM JA. Af-ter 50 days of culture new structures developed on leaves: buds, rhizoids, aposporous game-tophytes and intermediates. We found out that low JA concentrations promote development of buds, rhizoids and intermediates, while higher concentrations stimulate development of aposporous gametophytes. The largest number of sporofitic structures (buds and rhizoids) and intermediates developed on leaves with cutted adaxial epidermis, while development of aposporus gametophytes was largely accelerated on chopped leaves. Regeneration of leaf culture affected by exogenously added different concentrations of JA was similar to regene-ration, initiated in response to different levels of wounding.
Keywords:Platycerium bifurcatum, jasmonic acid, wounding, buds, rhizoids, aposporus gametophytes, intermediates


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