| | SLO | ENG | Cookies and privacy

Bigger font | Smaller font

Show document Help

Title:PREŽIVELOST SPOR EKSTREMOFILNIH GLIV IZPOSTAVLJENIH VISOKEMU HIDROSTATSKEMU TLAKU IN PRILAGODITEV NA USTVARJENE POGOJE
Authors:ID Recek, Nina (Author)
ID Knez, Željko (Mentor) More about this mentor... New window
ID Gunde Cimerman, Nina (Comentor)
Files:.pdf UNI_Recek_Nina_2011.pdf (4,12 MB)
MD5: CFB38E7D6B8B26D7FC14A4DBB11C8C13
PID: 20.500.12556/dkum/118e5b03-b4ff-42c7-ad26-97515a2c24cb
 
Language:Slovenian
Work type:Undergraduate thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Namen te diplomske naloge je določiti, kako izpostavitev spor izbranih ekstremofilnih vrst gliv visokemu hidrostatskemu tlaku vpliva na preživelost, rast, velikost in obliko glivnih kolonij po rasti na hranilnem mediju brez soli (MEA) ali na mediju z povečano koncentracijo soli (MEA+10% NaCl). Pri tem smo želeli preveriti ali prilagoditve gliv na višjo slanost in nizek vodni potencial v hranilnem mediju, lahko vpliva tudi na boljše preživetje njihovih spor izpostavljenih visokemu hidrostatskemu tlaku. Mehanizme prilagoditve, ki so bili predstavljeni kot odziv gliv na povišano koncentracijo soli v hranilnem mediju smo primerjali in poskušali povezati z mehanizmi prilagoditve za preživetje pod vplivom visokega hidrostatskega tlaka (HHP). Rezultati so pokazali, da so izpostavljene spore vseh gliv dobro prenesle 24 ur trajajočo izpostavitev 300 bar, saj je rast bila povsod zabeležena, neglede na hranilni medij. Izbrane glivne vrste so po predhodnem gojenju na mediju s povečano slanostjo (MEA+10% NaCl) bolje kompenzirale spremembe v turgorju, ki nastanejo zaradi izpostavitve visokemu hidrostatskemu tlaku. Eden izmed mehanizmov prilagoditve je sprememba razmerja med površino in volumnom spore ter združevanje v skupke. To so opazili in opisali kot mehanizem prilagoditve gliv na življenje v okoljih z visoko salnostjo in je verjetno eden izmed mehanizmov, ko se glive soočajo z visokim hidrostatskim tlakom, kar še ni bilo opisano v literaturi.
Keywords:ekstremofilne glive, spore, povišana slanost, visok hidrostatki tlak, mehanizmi prilagoditve, SEM
Place of publishing:Maribor
Publisher:[N. Recek]
Year of publishing:2011
PID:20.500.12556/DKUM-19223 New window
UDC:582.241(043.2)
COBISS.SI-ID:15158038 New window
NUK URN:URN:SI:UM:DK:UCM6YAGT
Publication date in DKUM:01.07.2011
Views:3432
Downloads:184
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
:
Copy citation
  
Average score:(0 votes)
Your score:Voting is allowed only for logged in users.
Share:Bookmark and Share



Hover the mouse pointer over a document title to show the abstract or click on the title to get all document metadata.

Secondary language

Language:English
Title:SURVIVAL OF SPORES OF EXTREMOPHILIC FUNGI EXPOSED TO HIGH HYDROSTATIC PRESSURE AND ADAPTATION TO CREATED CONDITIONS
Abstract:The aim of diploma thesis was to examine, how exposure of harvested conidia of selected extremophilic fungal species to high hydrostatic pressure, effects on survival, growth, size and shape of conidia when cultivated on media without salt (MEA) and on media with high concentration of salt (MEA+10%NaCl). Furthermore, we explore if pre-cultivation and adaptation of fungi on media with high salinity and consequently low water activity can influence on better survival of fungal conidia exposed to high hydrostatic pressure. Described mechanisms of adaptation of fungi to grow on media with high salinity was compared and linked with observed after exposure to high hydrostatic pressure (HHP). Results showed that fungal conidia well tolerated the long term exposure to 300bar as afterword growth was observed in all cases. Selected fungal species pre-cultivated on media with high salinity (MEA+10%NaCl) better tolerated the turgor caused by high hydrostatic pressure. One of the mechanisms of adaptation observed was change in surface to volume ratio and clamps formation. This was noticed and described as one of the adaptation mechanisms when fungi are coping with presence of high salinity in their environment and it might be also the case when exposed to high hydrostatic pressure not yet described in literature.
Keywords:extremophilic fungi, conidia, increased salinity, high hydrostatic pressure, mechanisms of adaptation, SEM


Comments

Leave comment

You must log in to leave a comment.

Comments (0)
0 - 0 / 0
 
There are no comments!

Back
Logos of partners University of Maribor University of Ljubljana University of Primorska University of Nova Gorica