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Title:PRIMERJAVA METOD DOLOČANJA CISTATINA C NA RAZLIČNIH ANALIZATORJIH
Authors:ID Senekovič, Mateja (Author)
ID Brodnjak-Vončina, Darinka (Mentor) More about this mentor... New window
ID Kolar, Mitja (Comentor)
ID Gorenjak, Maksimiljan (Comentor)
Files:.pdf UNI_Senekovic_Mateja_2011.pdf (3,11 MB)
MD5: 1CC6D8307C9CFB1DB92CEC2FFAFB8AFB
PID: 20.500.12556/dkum/5baa9857-cf1c-4e89-907e-3920c03b6611
 
Language:Slovenian
Work type:Undergraduate thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Cilj diplomske naloge je dokazati, ali nov analizator Siemens Dimension Vista 1500® daje primerljive rezultate nefelometru Siemens BN ProSpec. Na obeh analizatorjih smo merili koncentracije cistatina C v krvnem serumu z nefelometrično meritvijo, ki temelji na nastanku serumskih kompleksov med antigenom (cistatin C) in protitelesi. Analizirali smo 779 vzorcev zamrznjenega krvnega seruma v Univerzitetnem kliničnem centru Maribor. Metoda merjenja na analizatorju BN ProSpec velja kot ˝referenčna metoda˝, saj je daleč najbolj uveljavljena in se skoraj vsi laboratoriji zgledujejo po njej. Vista pa je prvi kompleksen analizator, ki ima dodatno vgrajeno nefelometrično metodo, je hitrejši ter priročnejši. Iz statističnega ovrednotenja rezultatov, statističnih testov, rezultatov linearnih regresijskih koeficientov, Passing Bablokove regresijske premice in Bland Altmanove metode lahko zaključimo, da je metoda, ki jo uporablja nov kompleksen analizator Siemens Dimension Vista 1500® primerljiva referenčni metodi, in da lahko analize cistatina C preselimo na nov instrument.
Keywords:cistatin C, nefelometrija, glomerulna filtracija
Place of publishing:Maribor
Publisher:[M. Senekovič]
Year of publishing:2011
PID:20.500.12556/DKUM-19902 New window
UDC:543
COBISS.SI-ID:15771926 New window
NUK URN:URN:SI:UM:DK:MFVCOJKO
Publication date in DKUM:07.02.2012
Views:3559
Downloads:364
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
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Secondary language

Language:English
Title:METHOD COMPARISON FOR DETERMINATION OF CYSTATIN C USING DIFFERENT ANALYSERS
Abstract:Aim of diploma thesis is to confirm, if the new analyser Siemens Dimension Vista 1500® gives comparable results with the nephelometer Siemens BN ProSpec. Concentrations of cystatin C in blood serum were measured on both analysers using nephelometric method, which is based on formation of serum complexes between antigen (cystatin C) and antibodies. 779 samples of frozen blood serum were analysed in University Medical Centre Maribor. Method performed on BN ProSpec analyser was chosen as the ˝reference method˝, as it is most usually used in nearly all laboratories. Vista analyser is the first complex analyser, with additionally incorporated nephelometric method. It is faster and more convenient for manipulation. From the statistical evaluation of measurements, namely statistical tests, linear regression coefficients, Passing Bablok regression method and Bland Altman method we can conclude that the new method which uses new complex Siemens Dimension Vista 1500® analyser is comparable with the reference method. From now on the analyses of cystatin C could be performed on the new instrument.
Keywords:cystatin C, nephelometry, glomerular filtration


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