PRIMERJAVA HITREGA IMUNOKEMIJSKEGA TESTA Z ELISA TESTOM ZA DOLOČITEV KALPROTEKTINA V BLATU

Krmpotić, Ivan

| | SLO | ENG | Cookies and privacy

Bigger font | Smaller font

First page > Show document

Show document

Title:	PRIMERJAVA HITREGA IMUNOKEMIJSKEGA TESTA Z ELISA TESTOM ZA DOLOČITEV KALPROTEKTINA V BLATU
Authors:	ID Krmpotić, Ivan (Author) ID Potočnik, Uroš (Mentor) More about this mentor... ID Krajnc, Peter (Mentor) More about this mentor... ID Homšak, Evgenija (Mentor) More about this mentor...
Files:	VS_Krmpotic_Ivan_2012.pdf (1,22 MB) MD5: 61855421EA6BBA30F32E02537EE02E5F PID: 20.500.12556/dkum/1dea5a09-955e-42f9-b725-89a65de795f6
Language:	Slovenian
Work type:	Undergraduate thesis
Typology:	2.11 - Undergraduate Thesis
Organization:	FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:	Kalprotektin v blatu je nespecifični marker črevesnega vnetja in eden izmed parametrov, ki so pomembni v diagnostiki kronične vnetne črevesne bolezni. Uporaben je predvsem pri razlikovanju med kronično vnetno črevesno boleznijo in sindromom razdražljivega črevesja (IBS). Namen naloge je bila primerjava hitrega imunokromatografskega testa z, v rutinski diagnostiki že uveljavljenim testom ELISA, za merjenje proteina kalprotektina v blatu. V delu smo primerjali rezultate semikvantitativne imunokromatografske metode z rezultati kvantitativne metode ELISA. Kot statistično orodje za primerjavo obeh testov med seboj smo uporabili vrednotenje z diagnostično občutljivostjo in diagnostično specifičnostjo. Preverili smo tudi ujemanje rezultatov glede na podane intervale hitrega testa z kvantitativnimi rezultati ELISA testa. Pri primerjavi obeh testov glede na koncentracijske razrede, smo ugotovili najvišje ujemanje pri nizkih koncentracijah (do 15 µg/g). Ujemanje glede na ELISA je bilo kar 97%. V razredu od 15 µg/g do 60 µg/g je bilo ujemanje 17%, nad 60 µg/g smo ugotovili 79% ujemanje. Ob predlagani mejni vrednosti ELISA 50 µg/g smo določili s statistično analizo 100% diagnostično občutljivost hitrega testa glede na ELISA, diagnostična specifičnost je bila 92,3%. Ko smo mejno vrednost ELISA povišali na 60 µg/g, se rezultati niso bistveno spremenili. Diagnostična občutljivost hitrega testa glede na ELISA je ostala 100%, diagnostična specifičnost je znašala 85,7%. Hitri test je primerljiv z ELISA pri opredeljevanju negativnih vzorcev, njegova kvantitativna zanesljivost pri nizkih koncentracijah (nivo kalprotektina pod 15 µg/g) nakazuje na njegovo uporabnost pri izključitvi suma na vnetni proces v črevesni sluznici. Če hitri test pokaže pozitiven rezultat je potrebna nadaljnja diagnostika, predvsem napotitev na testiranje z ELISA, saj hitri test ne izmeri koncentracij kalprotektina nad 60 µg/g. Poznavanje natančnih koncentracij v tem območju je pomembno pri bolnikovi obravnavi. Ob negativnem rezultatu vzorca blata ni potrebno pošiljati v diagnostični laboratorij na nadaljnje preiskave, ki bi bile povezane z ugotavljanjem prisotnosti črevesnega vnetja.
Keywords:	kalprotektin, ELISA, hitri test, KVČB, IBS, primerjava
Place of publishing:	Maribor
Publisher:	[I. Krmpotić]
Year of publishing:	2012
PID:	20.500.12556/DKUM-22036
UDC:	616.3(043.2)
COBISS.SI-ID:	16059926
NUK URN:	URN:SI:UM:DK:VYGOOITD
Publication date in DKUM:	23.02.2012
Views:	5388
Downloads:	542
Metadata:
Categories:	KTFMB - FKKT
:	Copy citation

Average score:	(0 votes)
Your score:	Voting is allowed only for logged in users.
Share:

Hover the mouse pointer over a document title to show the abstract or click on the title to get all document metadata.

Secondary language

Language:	English
Title:	A COMPARISON OF RAPID IMMUNOCHEMICAL TEST WITH ELISA TEST FOR DETERMINATION OF CALPROTECTIN IN FAECES
Abstract:	Faecal calprotectin is a non specific marker of intestinal inflammation and one of the parameters that are important in the diagnostics of inflammatory bowel disease (IBD). Its most important application is in its ability to distinguish between IBD and IBS (irritable bowel syndrome). The goal of our thesis was to compare a rapid immunochromatographic test with an ELISA test, which is already well established in routine diagnostics for determining levels of faecal calprotectin. In the thesis we compared the results of semikvantitative immunocromathographic method with kvantitative ELISA method. As a statistical tool for comparing both tests we used evaluation with diagnostic sensitivity and diagnostic specificity. We also compared the results of both tests in terms of given intervals of the rapid test with kvantitative results of ELISA. As we compared both tests in terms of concentration intervals we found highest accordance at the low concentrations (below 15 µg/g) where it was 97%. In the interval between 15 µg/g and 60 µg/g we found 17% accordance and in the interval above 60 µg/g accordance was 79%. At the suggested cut off value for ELISA 50 µg/g we got 100% diagnostic sensitivity of the rapid test compared to ELISA, diagnostic specificity was 92,3%. At elevated cut off value for ELISA, 60 µg/g there was no significant change in the test results. Diagnostic sensitivity of the rapid test compared to ELISA remained 100%, diagnostic specificity was 85,7%. In our thesis we find the rapid test to be comparable to ELISA in evaluation of negative samples, its kvantitative accurateness at low concentrations (below 15 µg/g) points to its usefulness at exclusion of suspected inflammatory process in the intestinal mucosa. Positive test result obtained with the rapid test must be followed by further diagnostic procedures, firstly measurment of calprotectin with ELISA, as the rapid test cannot measure concentrations above 60 µg/g. Knowing the exact values of calprotectin concentrations in this interval is crucial for treatment of the patient. If the rapid test gives a negative test result there is no need for sending the faecal sample to the laboratory for further examinations regarding intestinal inflammation.
Keywords:	calprotectin, ELISA, rapid test, IBD, IBS, comparison

Comments

Leave comment

You must log in to leave a comment.

Comments (0)

0 - 0 / 0

There are no comments!

Back