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Title:
Enzymatic reactions in dense gases
Authors:
ID
Knez, Željko
(
Author
)
Files:
http://dx.doi.org/10.1016/j.supflu.2008.11.012
Language:
English
Work type:
Unknown
Typology:
1.02 - Review Article
Organization:
FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:
The developments on applications of supercritical fluids as alternative solvents for biocatalytic processes that have taken place over the past two decades have been reviewed. An overview of process parameters influencing enzyme activity and stability, the influence of process parameters on reaction rates and productivity are presented. Applications of various types of reactors for enzymatic reaction in dense fluids, limitations of using enzymes as biocatalyst in supercritical fluids as well as future trends are presented. Main advantages of using dense gases as solvents for biocatalyzed reactions are the tunability of solvent properties and simple down stream processing features that can be readily combined with other unit operations. Although many enzymes are stable in supercritical fluids (SCFs) one should pay considerable attention to finding the correct reaction conditions for each substrate/enzyme/SCF system. One of the persistent problems is the instability and deactivation of enzymes under pressure and temperature. At present the most stable enzymes are hydrolases (lipases and esterases) for which pressure effect is lower than temperature deactivation.
Keywords:
biocatalysis
,
supercritical fluids
,
enzyme bioreactors
,
heterogeneous biocatalysis
Year of publishing:
2009
PID:
20.500.12556/DKUM-27351
UDC:
66-987
ISSN on article:
0896-8446
COBISS.SI-ID:
12947222
NUK URN:
URN:SI:UM:DK:LZQKVECF
Publication date in DKUM:
01.06.2012
Views:
2745
Downloads:
104
Metadata:
Categories:
Misc.
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Record is a part of a journal
Title:
The Journal of supercritical fluids
Shortened title:
J. supercrit. fluids
Publisher:
PRA Press
ISSN:
0896-8446
COBISS.SI-ID:
25795072
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