| Title: | Calcium dependencies of regulated exocytosis in different endocrine cells |
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| Authors: | ID Dolenšek, Jurij (Author)
ID Skelin, Maša (Author)
ID Rupnik, Marjan (Author) |
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| Files: |  http://www.biomed.cas.cz/physiolres/pdf/60%20Suppl%201/60_S29.pdf
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| Language: | English |
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| Work type: | Other |
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| Typology: | 1.02 - Review Article |
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| Organization: | MF - Faculty of Medicine
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| Abstract: | Exocytotic machinery in neuronal and endocrine tissues is sensitive to changesin intracellular Ca2+ concentration. Endocrine cell models, that are most frequently used to study the mechanisms of regulated exocytosis, are pancreatic beta cells, adrenal chromaffin cells and pituitary cells. To reliably study the Ca2+ sensitivity in endocrine cells, accurate and fast determination of Ca2+ dependence in each tested cell is required. With slow photo-release it is possible to induce ramp-like increase in intracellular Ca2+ concentration ([Ca2+]i) that leads to a robust exocytotic activity. Slow increases in the [Ca2+]i revealed exocytotic phases with different Ca2+ sensitivities that have been largely masked in step-like flash photo-release experiments. Strikingly, in the cells of the three described model endocrine tissues (beta, chromaffin and melanotroph cells), distinct Ca2+ sensitivity ćclassesć of secretory vesicles have been observed: a highly Ca2+-sensitive, amedium Ca2+-sensitive and a low Ca2+- sensitive kinetic phase of secretory vesicle exocytosis. We discuss that a physiological modulation of a cellular activity, e.g. by activating cAMP/PKA transduction pathway, can switch the secretory vesicles between Ca2+ sensitivity classes. This significantly alterslate steps in the secretory release of hormones even without utilizationof an additional Ca2+ sensor protein. |
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| Keywords: | Calcium sensitivity, Exocytosis, Insulin-secreting cells, Chromaffin cells, Melanotrophs |
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| Year of publishing: | 2011 |
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| PID: | 20.500.12556/DKUM-31136  |
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| UDC: | 576.32/.36 |
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| ISSN on article: | 0862-8408 |
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| COBISS.SI-ID: | 512147512 |
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| NUK URN: | URN:SI:UM:DK:RKH90VWC |
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| Publication date in DKUM: | 05.06.2012 |
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| Views: | 2294 |
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| Downloads: | 94 |
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| Metadata: |    |
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| Categories: | Misc.
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