| | SLO | ENG | Cookies and privacy

Bigger font | Smaller font

Show document Help

Title:VPLIV SUPERKRITIČNEGA OGLIKOVEGA DIOKSIDA NA AKTIVNOST ZAMREŽENIH ENCIMSKIH AGREGATOV (CLEAS) IZ CELULAZE
Authors:ID Gselman, Doroteja (Author)
ID Leitgeb, Maja (Mentor) More about this mentor... New window
ID Primožič, Mateja (Comentor)
Files:.pdf UNI_Gselman_Doroteja_2012.pdf (1,82 MB)
MD5: C595FD1BBF431150A4CDDED3A2E2D66E
PID: 20.500.12556/dkum/7c31a372-7142-4545-9bf5-c053a208b8aa
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Namen diplomskega dela je bil določiti vpliv superkritičnega ogljikovega dioksida (SC CO2) na aktivnost zamreženih encimskih agregatov (CLEAs) iz encima celulaze. CLEAs in prosti encim celulaze smo v visokotlačnem šaržnem reaktorju izpostavili SC CO2 pri temperaturi 50°C in tlaku 200 bar. Istočasno smo CLEAs in prosto celulazo inkubirali pri atmosferskem tlaku in 50°C. Iz rezultatov je razvidno, da se je aktivnost CLEAs s podaljševanjem časa izpostavljanja v SC CO2 zviševala medtem, ko se je aktivnost pri izpostavitvi CLEAs v atmosferskemu tlaku znižala. Določili smo tudi koncentracijo proteinov CLEAs in proste celulaze. S podaljševanjem časa izpostavitve v SC CO2 se je koncentracija proteinov zvišala. Optimirali smo različne parametre pri pripravi CLEAs iz celulaze. Najvišja aktivnost CLEAs je bila dosežena, ko smo albumin razredčili v PBS pufru s koncentracijo 0,02 M.
Keywords:CLEAs, SC CO2, celulaza, atmosferski tlak, proteini
Place of publishing:Maribor
Publisher:[D. Gselman]
Year of publishing:2012
PID:20.500.12556/DKUM-38613 New window
UDC:577.15:[66-9874-139](043.2)
COBISS.SI-ID:16683030 New window
NUK URN:URN:SI:UM:DK:TJCCWMVY
Publication date in DKUM:16.10.2012
Views:2424
Downloads:232
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
:
Copy citation
  
Average score:(0 votes)
Your score:Voting is allowed only for logged in users.
Share:Bookmark and Share



Hover the mouse pointer over a document title to show the abstract or click on the title to get all document metadata.

Secondary language

Language:English
Title:INFLUENCE OF SUPERCRITICAL CARBON DIOXIDE ON ACTIVITY CROSS-LINKED ENZYME AGGREGATES OF CELLULASE
Abstract:The purpuse of the diploma thesis is to examine the effect of supercritical carbon dioxide (SC CO2) on the activity of cross-linked enzyme aggregates from cellulase. CLEAs and pure enzyme cellulase were exposed to SC CO2 in high pressure batch reactor at 50 °C and 200 bar. At the same time CLEAs and free cellulase were exposed to atmospheric pressure at 50 °C. The results showed that activity of CLEAs in dependance of incubation time in SC CO2 increased, meanwhile the activity of CLEAs in atm. pressure decreased. The concentration of proteins in CLEAs and free cellulase was also measured. In prolongation of incubation time in SC CO2 the concentration of proteins in CLEAs and free cellulase increased. Different parameters was optimized in preparation of CLEAs from cellulase. The highest activity of CLEAs was obtained when the albumin was diluted in PBS buffer with the concentration 0,02 M.
Keywords:CLEAs, SC CO2, cellulase, atmospheric pressure, proteins


Comments

Leave comment

You must log in to leave a comment.

Comments (0)
0 - 0 / 0
 
There are no comments!

Back
Logos of partners University of Maribor University of Ljubljana University of Primorska University of Nova Gorica