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Title:VPLIV SUPERKRITIČNEGA OGLJIKOVEGA DIOKSIDA NA IZLOČANJE PROTEINOV IN NUKLEINSKIH KISLIN IZ GLIVE HORTAEA WERNECKII
Authors:ID Smej, Diana (Author)
ID Leitgeb, Maja (Mentor) More about this mentor... New window
Files:.pdf UNI_Smej_Diana_2012.pdf (2,92 MB)
MD5: EA247A39C75B04DE755060E57C144EED
PID: 20.500.12556/dkum/f3259eb4-381c-4b99-a705-9a2f6c8898e8
 
Language:Slovenian
Work type:Undergraduate thesis
Typology:2.11 - Undergraduate Thesis
Organization:FNM - Faculty of Natural Sciences and Mathematics
Abstract:Hortaea werneckii je zaradi izjemne tolerantnosti na slana območja, postala evkariontski model organizma za prilagoditev na slanost. Različne študije so dokazale, da ima ta gliva več mehanizmov za prilagoditev na zelo slana območja. Superkritični ogljikov dioksid se že nekaj časa uporablja v medicini in prehrambni industriji za sterilizacijo materialov in za zmanjševanje aktivnosti nekaterih organizmov, kot so bakterije in glive. Pri visokih tlakih lahko povzroči celično smrt, pri nižjih tlakih pa poškoduje celice do te mere, da se intracelične spojine in strukture izločijo iz celice. Cilj diplomske naloge je bil določiti preživelost glive H. werneckii po izpostavljanju v SC CO2, ugotoviti, kako le-ta vpliva na aktivnost izločenih encimov in koliko proteinov in nukleinskih kislin se bo izločilo po tem, ko smo glivo izpostavili v SC CO2 pri tlaku 100 in 300 bar in različnih časovnih obdobjih.
Keywords:Hortaea werneckii, superkritični ogljikov dioksid, aktivnost encimov, proteini, nukleinske kisline
Place of publishing:Maribor
Publisher:[D. Smej]
Year of publishing:2012
PID:20.500.12556/DKUM-39256 New window
UDC:54(043.2)
COBISS.SI-ID:19591432 New window
NUK URN:URN:SI:UM:DK:6HWTYF7N
Publication date in DKUM:04.01.2013
Views:2750
Downloads:195
Metadata:XML DC-XML DC-RDF
Categories:FNM
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Secondary language

Language:English
Title:INFLUENCE OF SUPERCRITICAL CARBON DIOXIDE ON THE SECRETION OF PROTEINS AND NUCLEIC ACID FROM FUNGI HORTAEA WERNECKII
Abstract:Hortaea werneckii is due to the exceptional tolerance to saltwater areas become eukaryotic model organism for adaptation to salinity. Various studies have demonstrated that this fungus has several mechanisms to adapt to highly saline areas. Supercritical carbon dioxide is already used in medicine and food industry to sterilize materials and to reduce the activities of some organisms, such as bacteria and fungi. At high pressures can cause cell death and at lower pressures damaged cells to such an extent, that the intracellular compounds and the structure extracted from the cell. The purpose of diploma work paper was to determine the survival of fungus H. werneckii after exposure in SC CO2, determine how it will affect on activity of eliminated enzymes and how many proteins and nucleic acids will be excreted after we are expose H. werneckii in SC CO2 at a pressure of 100 and 300 bar and different time periods.
Keywords:Hortaea werneckii, supercritical carbon dioxide, activity of enzymes, proteins, nucleic acids


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