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Title:Imobilizacija encima celulaza v zamrežene encimske skupke (CLEAs) ter vpliv superkritičnega ogljikovega dioksida na njihovo aktivnost
Authors:ID Pogorelc, Katja (Author)
ID Leitgeb, Maja (Mentor) More about this mentor... New window
Files:.pdf UNI_Pogorelc_Katja_2014.pdf (1,31 MB)
MD5: 638429AF771C4A6BACF606091193E167
 
Language:Slovenian
Work type:Undergraduate thesis
Typology:2.11 - Undergraduate Thesis
Organization:FNM - Faculty of Natural Sciences and Mathematics
Abstract:Diplomsko delo opisuje sintezo encimskih skupkov iz encima celulaza ali na kratko CLEAs ter vpliv superkritičnega ogljikovega dioksida (SC CO2) na aktivnost teh skupkov. Priprava zamreženih encimskih skupkov poteka po določenem postopku, katerega ključna dela sta obarjanje in zamreženje. Torej obarjanje topnega encima z ustreznim organskim topilom in nadaljnjo zamreženje tako oborjenega encima s pomočjo mrežnega povezovalca. V našem primeru smo kot mrežni povezovalec uporabili glutaraldehid in aceton kot obarjalni reagent. Zamrežene encimske skupke (CLEAs) smo izpostavili pri temperaturah 35 °C, 40 °C, 50 °C in 60 °C. Istočasno smo jih izpostavili v SC CO2 v visokotlačnem šaržnem reaktorju, in sicer pri temperaturah 35 °C, 40 °C, 50 °C in 60 °C in tlaku 100 bar ter pri temperaturah 35 °C in 40 °C ter tlaku 200 bar. Aktivnost CLEAs je s podaljšanjem inkubacijskega časa upadala.
Keywords:sinteza encimskih skupkov (CLEAs), celulaza, obarjenje, zamreženje, mrežni povezovalec, glutaraldehid, superkritični ogljikov dioksid
Place of publishing:Maribor
Publisher:[K. Pogorelc]
Year of publishing:2014
PID:20.500.12556/DKUM-44510 New window
UDC:577.15(043.2)
COBISS.SI-ID:20611848 New window
NUK URN:URN:SI:UM:DK:H3TALBAC
Publication date in DKUM:18.06.2014
Views:2327
Downloads:244
Metadata:XML DC-XML DC-RDF
Categories:FNM
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Secondary language

Language:English
Title:Cellulase immobilization as cross linked enzyme aggregates (CLEAs) and influence on enzyme activity of supercritical carbon dioxide
Abstract:This undergraduate thesis examines the synthesis of cross-linked enzyme aggregates or CLEAs from cellulase and the impact of supercritical carbon dioxide (SC CO2) on the activity of these aggregates. Cross-linked enzyme aggregates are produced using a special procedure composed of two key stages: precipitation and cross-linking. The soluble enzyme is precipitated with a suitable organic solvent and the enzyme precipitated in this manner is subsequently cross-linked using a cross-linking agent. In this study, acetone was used as the precipitant and glutaraldehyde as the cross-linker. CLEAs were exposed to atmospheric pressure at various temperatures (35 °C, 40 °C, 50 °C, and 60 °C). They were also exposed to SC CO2 in a high-pressure batch reactor at 35 °C, 40 °C, 50 °C, and 60 °C and 100-bar pressure, and at 35°C and 40 °C and 200-bar pressure. Exposing the CLEAs to various temperatures and pressures for various periods of time revealed that the activity of aggregates decreases with time.
Keywords:synthesis of enzyme aggregates (CLEAs), cellulase, precipitation, cross-linking, cross-linking agent, glutaraldehyde, supercritical carbon dioxide


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