IMOBILIZACIJA KOMERCIALNEGA ENCIMSKEGA PREPARATA DENILITE II S

Slavič, Anja

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Title:	IMOBILIZACIJA KOMERCIALNEGA ENCIMSKEGA PREPARATA DENILITE II S
Authors:	ID Slavič, Anja (Author) ID Primožič, Mateja (Mentor) More about this mentor... ID Leitgeb, Maja (Comentor)
Files:	UNI_Slavic_Anja_2014.pdf (2,22 MB) MD5: B18C60595B06B434CCECAAC2BB22EC86
Language:	Slovenian
Work type:	Bachelor thesis/paper
Typology:	2.11 - Undergraduate Thesis
Organization:	FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:	Namen diplomske naloge je bila uspešna imobilizacija komercialnega encimskega preparata DENILITE II S na hitozanske maghemitne nanodelce in primerjava aktivnosti imobiliziranega encima s prostim encimom. Sintetizirali smo hitozanske maghemitne nanodelce po metodi kovalentne vezave in nanje imobilizirali encim. Za aktivacijo funkcionalnih skupin nosilca smo uporabili mrežna povezovalca glutaraldehid (GA) in pentaetilen heksamin (PEHA). Z različnimi koncentracijami mrežnega povezovalca smo aktivirali funkcionalne skupine nosilca ter iskali optimalno učinkovitost imobilizacije in preostalo aktivnost imobiliziranega encima. Zanimala nas je tudi stabilnost encima, kako vplivata čas in temperatura izpostavitve na ohranitev preostale aktivnosti imobiliziranega encima. Imobilizirani encim ima prednost pred prostim encimom, saj se lahko večkrat uporabi, zato smo preverili, kako vpliva večkratna uporaba na ohranitev preostale aktivnosti imobiliziranega encima. Najvišjo preostalo aktivnost imobiliziranega encima na hitozanske maghemitne nanodelce smo dosegli pri dodatku mrežnega povezovalca GA (3 %) in koncentraciji encima 0,05 g/mL. Encim, imobiliziran na hitozanske maghemitne nanodelce pri optimalnih pogojih, smo izpostavili za določen čas različnim temperaturam in opazili, da se preostala aktivnost ne ohrani. Proučevali smo tudi vpliv večkratne uporabe imobiliziranega encima na preostalo aktivnost. Imobiliziran encim smo večkrat ponovno uporabili in ugotovili, da aktivnost encima z naraščanjem števila ciklov upada.
Keywords:	nanotehnologija, nanomateriali, hitozanski maghemitni nanodelci, imobilizacija encima, encim lakaza, aktivnost encima.
Place of publishing:	Maribor
Publisher:	[A. Slavič]
Year of publishing:	2014
PID:	20.500.12556/DKUM-45225
UDC:	543.645.4:620.3(043.2)
COBISS.SI-ID:	18248726
NUK URN:	URN:SI:UM:DK:4INUYO0D
Publication date in DKUM:	09.09.2014
Views:	2335
Downloads:	178
Metadata:
Categories:	KTFMB - FKKT
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Secondary language

Language:	English
Title:	IMMOBILIZATION OF COMMERCIAL ENZYME PREPARATION DENILITE II S
Abstract:	The goal of the thesis was to successfully immobilise DENILITE II S, a commercial enzyme preparation, onto maghemite nanoparticles coated by chitosan and to compare the activity of the immobilised enzyme to that of the free enzyme. The chitosan coated nanoparticles had been synthesised with the covalent bond method and the immobilised enzyme was added. To activate the functional groups of the carrier, glutaraldehyde (GA) and pentaethylenehexamine (PEHA) were used as crosslinking agents. By applying different concentrations of the crosslinking agents, functional groups of the carrier were activated and looked to determine the optimal efficiency of the enzyme immobilisation and the remaining activity of the immobilised enzyme. We were also interested in the stability of the enzyme and how the remaining activity of the immobilised enzyme was affected by exposed time and temperature. The immobilised enzyme has an advantage over the free enzyme, because it can be used more times; therefore we examined how multiple usage affects the remaining activity of the immobilised enzyme. The highest values in the remaining activity of the enzyme immobilised onto maghemite nanoparticles coated by chitosan was achieved, when the crosslinking agent GA (3%) was applied and with enzyme concentration at 0.05 g/mL. The enzyme, immobilised onto chitosan coated nanoparticles under optimal conditions, was exposed to different temperatures for a certain period of time and we observed that the remaining activity does not remain preserved. The effect of multiple usage of the immobilised enzyme on its remaining activity was also examined. The immobilised enzyme was reused several times and the remaining activity of the enzyme decreased with the number of cycles.
Keywords:	nanotechnology, nanomaterials, chitosan-coated maghemite nanoparticles, enzyme immobilization, laccase enzyme, enzyme activity.

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