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Title:DOLOČANJE ANIONOV V REALNIH VZORCIH Z IONSKO KROMATOGRAFIJO
Authors:ID Hotko, Anka (Author)
ID Kolar, Mitja (Mentor) More about this mentor... New window
ID Kristl, Janja (Comentor)
Files:.pdf UNI_Hotko_Anka_2014.pdf (2,87 MB)
MD5: 28C225C38AC8052C7B6A69ADFFDF36B2
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Namen diplomskega dela je bila optimizacija analizne metode po ekstrakciji, s katero smo določili vsebnost kloridnih, nitratnih in oksalatnih anionov v ekstraktu tara (Colocasia esculenta (L.) Schott)) s pomočjo ionske kromatografije (IC). Ekstrakcijo smo optimirali glede: načina izvedbe, temperature in učinkovitosti Tween-reagenta. Za realni vzorec, ekstrakt tara, smo študirali sestavo mobilne faze in sicer tako, da smo dobili zadovoljivo ločljivost pikov, ki jih želimo kvantitativno ovrednotiti. Analizno metodo smo tudi delno validirali, saj smo določili: linearnost, mejo zaznavnosti, mejo določljivosti, natančnost, pravilnost, vpliv matrice in časovno stabilnost standardov. V realnih vzorcih tara, smo določili vsebnost kloridnih, nitratnih in oksalatnih ionov v koncentracijskem območju od 197 mg/kg do 2 930 mg/kg SS, od 11 mg/kg do 10 868 mg/kg SS in od 761 mg/kg do 2 680 mg/kg SS.
Keywords:IC, anioni, validacija, realni vzorci
Place of publishing:Maribor
Publisher:[A. Hotko]
Year of publishing:2014
PID:20.500.12556/DKUM-45782 New window
UDC:543.354-128.2(043.2)
COBISS.SI-ID:18237974 New window
NUK URN:URN:SI:UM:DK:ALMIMFQG
Publication date in DKUM:22.09.2014
Views:2005
Downloads:212
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
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Secondary language

Language:English
Title:DETERMINATION OF ANIONS IN REAL SAMPLES USING ION CHROMATOGRAPHY
Abstract:The purpose of the diploma thesis was the optimisation of analytical method, for the determination of chloride, nitrate and oxalate anions content in taro (Colocasia esculenta (L.) Schott) extracts usng ion chromatography (IC). The extraction was optimised with regard to manner of performance and extraction temperature. Teh efficiency of Tween 80-reagent for the extraction of nitrate ions was also studied. We optimised the composition of the mobile phase for a real extract of taro sample in order to obtain complete separation od chromatographic peaks for anions of interest. The analytical method was also partly validated. We determined: linearity, limit of detection and quantification, precision, trueness, matrix influence and temporal stability of standard solutions. The contents of chloride, nitrate and oxalate ions were in the ranges from 197 mg/kg to 2 930 mg/kg DM, 11 mg/kg to 10 868 mg/kg DM and 761 mg/kg to 2 680 mg/kg DM, respectively
Keywords:IC, anions, validation, real samples


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