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Title:PRIDOBIVANJE MAGNETNIH ZAMREŽENIH ENCIMSKIH SKUPKOV S PRECIPITACIJO Z ETANOLOM
Authors:ID Matis, Staša (Author)
ID Primožič, Mateja (Mentor) More about this mentor... New window
ID Leitgeb, Maja (Comentor)
Files:.pdf UN_Matis_Stasa_2015.pdf (1,87 MB)
MD5: 03DF60FCBD092AD5273ED64CB0CFC195
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Diplomsko delo zajema pripravo magnetnih zamreženih encimskih skupkov (ali na kratko mCLEAs) iz encima celulaze. Naš namen je bil določiti optimalne pogoje za pripravo mCLEAs tako, da bosta učinkovitost in aktivnost imobilizacije zamreženih encimskih skupkov najvišja. V prvem delu diplomskega dela smo pripravili magnetne zamrežene encimske skupke s precipitacijo z etanolom. Pri tem smo spreminjali različne parametre (hitrost stresanja, čas stresanja, koncentracijo glutaraldehida in temperaturo), medtem ko je bil namen drugega dela diplomskega dela izpostavitev mCLEAs različnim temperaturam (23°C, 4°C, -15°C), ter ugotavljanje, kako se aktivnost mCLEAs pri različnih pogojih izpostavitve spreminja s časom. Naši rezultati prikazujejo, da je bila aktivnost mCLEAs najvišja, ko smo si mCLEAs pripravili pri 10°C in uporabili 0,7 % koncetracijo glutaraldehida. Pri izpostavitvi mCLEAs različnim temperaturam pa smo ugotovili, da aktivnost mCLEAs s časom najbolj upada kadar so bili mCLEAs izpostavljeni pri -15°C.
Keywords:etanol, glutaraldehid, magnetni zamreženi encimski skupki, precipitacija, zamreženje
Place of publishing:Maribor
Publisher:[S. Matis]
Year of publishing:2015
PID:20.500.12556/DKUM-54412 New window
UDC:602.4.577.151.4(043.2)
COBISS.SI-ID:19424790 New window
NUK URN:URN:SI:UM:DK:3PAUTF35
Publication date in DKUM:22.10.2015
Views:1841
Downloads:175
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
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Secondary language

Language:English
Title:SYNTHESIS OF MAGNETIC CROSS-LINKED ENZYME AGGREGATES WITH ETHANOL PRECIPITATION
Abstract:The aim of this work was to prepare the magnetic cross-linked enzyme aggregates (or briefly mCLEAs) of the enzyme cellulase. Our goal was to optimize the preparation of mCLEAs so that the efficiency and activity of the immobilized cross-linked enzyme would be preserved. In the first part of the thesis, we used ethanol precipitation to prepare magnetic cross-linked enzyme aggregates. In this part we tested how different condition (agitation rate, the agitation time, the concentration of glutaraldehyde, and temperature) affect mCLEAS activity. In the second part of the thesis we tested how different temperatures that we used during mCLEAs preparation (23 ° C, 4 ° C, -15 ° C) affect the final mCLEAs activity. Our results show that we obtain the highest final mCLEAs activity when we are working at 10°C and using 0.7% glutaraldehyde. The highest decrease in mCLEAs activity we obtained when mCLEAs were exposed to -15 ° C.
Keywords:ethanol, glutaraldehyde, magnetic cross-linked enzyme aggregates, precipitating, crosslinking


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