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Title:IN VITRO RAZMNOŽEVANJE ŽAJBLJA (Salvia officinalis L.)
Authors:ID Bencik, Dalia (Author)
ID Šiško, Metka (Mentor) More about this mentor... New window
Files:.pdf VS_Bencik_Dalia_2016.pdf (1,13 MB)
MD5: 3F381F3374015B39DA54F2EE62B0B7F7
 
Language:Slovenian
Work type:Bachelor thesis/paper
Organization:FKBV - Faculty of Agriculture and Life Sciences
Abstract:V letih 2012–2014 smo v in vitro razmerah s tehniko tkivnih kultur razmnoževali žajbelj (Salvia officinalis L.). V poskus smo vključili izsečke S. officinalis kot izhodiščni material za gojenje v in vitro razmerah ter jih dali na indukcijsko gojišče. Rastlinski material smo z uporabo petih različnih metod sterilizacije predhodno površinsko sterilizirali. Najbolj uspešna je bila sterilizacija pri 15 minutnem obravnavanju s 50% etanolom in DICO. Druga naloga raziskave je bila najti čim bolj primerno gojišče za uspešno razmnoževanje žajblja. Pripravili smo tri različna gojišča G1, G2 in G3, ki so se med seboj razlikovala po vsebnosti rastnih regulatorjev. Največ poganjkov smo namnožili na gojišču G2, ki je vsebovalo BAP v koncentraciji 1 mg/l in IAA v koncentraciji 0,5 mg/l. Tretji del raziskave pa je bil povezan s koreninjenjem. Uporabili smo dve različni gojišči, K1 in K2. Število ukoreninjenih rastlin pri K1 je predstavljalo 82 %, pri K2 pa 79 % vseh rastlin. Na obeh gojiščih smo dobili dobre rezultate. V zaključnem delu raziskave smo rastline aklimatizirali v mini rastlinjakih. Po enem mesecu opazovanja smo prišli do zaključka, da so bile rastline uspešno aklimatizirane.
Keywords:mikropropagacija, in vitro, tkivne kulture, žajbelj, Salvia officinalis L
Place of publishing:Maribor
Year of publishing:2016
PID:20.500.12556/DKUM-58080 New window
NUK URN:URN:SI:UM:DK:ZWYZEC9O
Publication date in DKUM:22.04.2016
Views:2586
Downloads:328
Metadata:XML DC-XML DC-RDF
Categories:FKBV
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Secondary language

Language:English
Title:IN VITRO PROPAGATION OF THE COMMON SAGE (Salvia officinalis L.)
Abstract:Between 2012 and 2014 we applied the in vitro tissue culture technique to reproduce common sage (Salvia officinalis L.). The experiment involved ordinary cuts/fragments of S. officinalis as starting material for the in vitro cultivation, which was placed on the induction culture medium. The surface of the involved plant material was pre-sterilized using five different approaches. The most successful sterilization involved 50% ethanol and DICA (sodium dichloroisocyanurat) and lasted 15 min. The second task of the experiment was to find the most appropriate medium for sage reproduction. We prepared three different culture media: G1, G2 and G3, each with a different content of growth regulators. Most of the shoots were multiplied in the culture medium G2 that contained BAP (benylamino purine) in concentration of 0.5 mg/l. The aim of the third task was determine the best medium for rooting. We used two different culture media: K1 and K2. The number of rooted plants in the K1 culture medium represents 82% and in K2 79% of all plants. We got good results on both K1 and K2. The last task was the acclimatisation of plantlets in a miniature greenhouse. After a month of observations, we came to the conclusion that the majority of plants were successfully acclimatised.
Keywords:micropropagation, in vitro, tissue culture, common sage, Salvia officinalis L.


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