ODPIRANJE CELIC HALOFILNE GLIVE HORTAEA WERNECKII S HOMOGENIZATORJEM IN ZASLEDOVANJE AKTIVNOSTI PRISOTNIH ENCIMOV

Švigelj, Jadranka

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Title:	ODPIRANJE CELIC HALOFILNE GLIVE HORTAEA WERNECKII S HOMOGENIZATORJEM IN ZASLEDOVANJE AKTIVNOSTI PRISOTNIH ENCIMOV
Authors:	ID Švigelj, Jadranka (Author) ID Leitgeb, Maja (Mentor) More about this mentor... ID Čolnik, Maja (Comentor)
Files:	UN_Svigelj_Jadranka_2016.pdf (2,52 MB) MD5: EDA674FD1E5773CF21D996E045999009
Language:	Slovenian
Work type:	Bachelor thesis/paper
Typology:	2.11 - Undergraduate Thesis
Organization:	FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:	Črna kvasovka Hortaea werneckii je ekstremofilna gliva, ki uspeva v širokem spektru slanosti, in sicer od 0 % do 32 % NaCl. Tako je H. werneckii zelo zanimiva z vidika odzivanja in prilagajanja na spremembe zunanjega okolja. Za odpiranje celic H. werneckii smo uporabili mehansko metodo homogenizacije. S to metodo poškodujemo celično steno in membrano, da se vsebina celice sprosti v celični medij. Namen našega dela je bil ugotoviti vpliv časa homogenizacije na različne dejavnike, in sicer na preživelost celic H. werneckii, preostalo aktivnost encimov (proteaza, α-amilaza, β glukozidaza in celulaza) in preostalo koncentracijo proteinov, ki smo jo določili z Bradfordovo metodo. Določevali smo tudi razmerje absorbanc nukleinskih kislin pri 260 nm in proteinov pri 280 nm s pomočjo UV-Vis sprektrofotometra. Celično suspenzijo H. werneckii smo homogenizirali do 100 min pri 35 °C. Ugotovili smo, da preživelost H. werneckii s podaljševanjem časa homogenizacije upada, preostala koncentracija proteinov v celični suspenziji H. werneckii pa narašča.
Keywords:	H. werneckii, encimi, proteini, mehanske metode, črne glive
Place of publishing:	Maribor
Publisher:	[J. Švigelj]
Year of publishing:	2016
PID:	20.500.12556/DKUM-61808
UDC:	575.853(043.2)
COBISS.SI-ID:	20341014
NUK URN:	URN:SI:UM:DK:B2LJHNMG
Publication date in DKUM:	16.09.2016
Views:	2484
Downloads:	152
Metadata:
Categories:	KTFMB - FKKT
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Secondary language

Language:	English
Title:	CELL DISRUPTION OF HALOPHILIC FUNGI HORTAEA WERNECKII WITH HO0MOGENIZER AND ENZYME ACTIVITY MEASUREMENTS
Abstract:	Black yeast Hortaea werneckii is an extremophilic fungi, which can thrive in a wide range of salinity from 0 % to 32 % NaCl. Thus, H. werneckii is very interesting in terms of responding and adapting to changes in the environment. To open the cells of H. werneckii, a mechanical homogenization method was used. With this method, the cell wall and membrane are damaged. Therefore, the cell contents are released into the cell medium. The purpose of our work was to determine the effect of homogenization time on various factors, that is to say on the viability of H. werneckii cells, residual activity of enzymes (protease, α-amylase, β glucosidase and cellulase), and the residual concentration of proteins, which was determined by the Bradford method. We also determined the ratio of the absorbance of the nucleic acids at 260 nm and proteins at 280 nm using an UV-Vis spectrophotometer. Homogenization of cell suspension of H. werneckii was performed for 100 min at 35 °C. We found that the viability of H. werneckii with increasing time of homogenization reduces and that the residual protein concentration increases.
Keywords:	H. werneckii, enzymes, proteins, mechanical methods, black yeasts

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