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Title:Prisotnost različnih genotipov bakterije Clostridium difficile pri hospitaliziranih bolnikih v Sloveniji med dvomesečnim zimskim obdobjem
Authors:ID Rupnik, Maja (Author)
ID Beigot Glaser, Sara (Author)
ID Andlovic, Alenka (Author)
ID Berce, Ingrid (Author)
ID Žohar Čretnik, Tjaša (Author)
ID Drinovec, Bojan (Author)
ID Harlander, Tatjana (Author)
ID Orešič, Nadja (Author)
ID Ravnik, Mateja (Author)
ID Štrumbelj, Iztok (Author)
Files:.pdf Zdravniski_vestnik_2013_Rupnik_et_al._Prisotnost_razlicnih_genotipov_bakterije_Clostridium_difficile_pri_hospitaliziranih_bolnikih_v_Slo.pdf (92,31 KB)
MD5: 93D943FE7A2D49395B87CCD1B8B17400
 
URL http://vestnik.szd.si/index.php/ZdravVest/article/view/990
 
Language:Slovenian
Work type:Scientific work
Typology:1.01 - Original Scientific Article
Organization:MF - Faculty of Medicine
Abstract:Izhodišča: Clostridium difficile je trenutno v svetu med najpomembnejšimi povzročitelji bolnišničnih črevesnih okužb. Seve lahko nadalje razlikujemo z ribotipizacijo. Nekateri ribotipi (npr. 027) so v številnih evropskih državah povezani z obširnimi izbruhi, povišano obolevnostjo in povišano smrtnostjo. V tem prispevku opisujemo razširjenost različnih ribotipov na območju celotne Slovenije. Metode: Seve bakterije C. difficile, ki so jih osamili v vseh osmih laboratorijih, kjer v Sloveniji diagnosticirajo bakterijo C. difficile, smo ribotpizirali. Prav tako smo zbrali podatke o skupnem številu opravljenih preiskav na C. difficile ter demografske podatke (starost in spol bolnikov). Rezultati: V dvomesečnem zimskem obdobju je bilo v Sloveniji testiranih skupaj 860 vzorcev, od teh je bilo pozitivnih 154 (17,9 %) vzorcev pri 125 bolnikih. Odstotek pozitivnih vzorcev v posameznem laboratoriju se je gibal od 13,3 do 43,2 %. Dva od osmih laboratorijev (oba iz primorsko-obalne regije) v času študije nista imela pozitivnih vzorcev. Tipiziranih je bilo 149 sevov. Uvrstili smo jih v 35 ribotipov, vendar je bilo več kot polovica sevov (57,7 %) v samo dveh ribotipih, 027 in 014/020. Ribotip 027 v Sloveniji do leta 2010 ni bil zaznan, v tej študiji je bil najpogostejši ribotip, prevladoval pa je v severovzhodni regiji. Zaključki: Raznolikost sevov C. difficile v Sloveniji je velika. Prevladovanje ribotipa 027 in visok odstotek pozitivnih vzorcev v nekaterih regijah kažejo na možnost izbruhov okužbe.
Keywords:Clostridium difficile, bolnišnične okužbe, mikrobiološka diagnostika
Publication status:Published
Publication version:Version of Record
Year of publishing:2013
Number of pages:str. 739-745
Numbering:Letn. 82, št. 11
PID:20.500.12556/DKUM-65265 New window
ISSN:1318-0347
UDC:616-07
ISSN on article:1318-0347
COBISS.SI-ID:31093721 New window
NUK URN:URN:SI:UM:DK:SNMVDYQU
Publication date in DKUM:27.03.2017
Views:1481
Downloads:164
Metadata:XML DC-XML DC-RDF
Categories:Misc.
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Record is a part of a journal

Title:Zdravniški vestnik. glasilo Slovenskega zdravniškega društva
Publisher:Slovensko zdravniško društvo
ISSN:1318-0347
COBISS.SI-ID:32893696 New window

Licences

License:CC BY-NC 4.0, Creative Commons Attribution-NonCommercial 4.0 International
Link:http://creativecommons.org/licenses/by-nc/4.0/
Description:A creative commons license that bans commercial use, but the users don’t have to license their derivative works on the same terms.
Licensing start date:27.03.2017

Secondary language

Language:English
Title:Diversity of C. difficile PCR ribotypes isolated from hospitalised patients in Slovenia during two-winter-month period
Abstract:Background: Clostridium difficile is an important cause of nosocomial diarrhoea. Strains arefurther differentiated into PCR ribotypes, andsome ribotypes (e.g. 027) are often associated with outbreaks, increased disease severity and increased mortality. Here we describe the diversity of C. difficile among human isolates in Slovenia. Methods: All eight microbiological diagnostic laboratories providing C. difficile diagnostics in Slovenia have participated. Isolates from two month- winter period were collected and ribotyped.The following data were also collected from the laboratories: number of all tested samples, number of all positive samples, and patient age and gender. Results: In a two-month period, altogether 860 samples were tested for C. difficile in all participating laboratories. Of those, 154 (17.9 %) samples from 125 patients were positive. The percentage of positive samples in different laboratories ranged from 13.3 to 43.2%. Two out of eight laboratoriesdid not have positive samples. C. difficile strains(n= 149) were grouped into 35 ribotypes. However, 57.7% of all strains belonged only to two PC Rribotypes (027 and 014/020). PCR ribotype 027 was not present in Slovenia until 2010, but was in this study the most prevalent PCR ribotype and present mainly in the northeast region. Conclusions: There is a substantial diversity of C. difficile ribotypes in Slovenia. A high prevalence of ribotype 027 and a high percentage of positive samples in some laboratories indicate the presence of C. difficile outbreaks.
Keywords:Clostridium difficile, nosocomial infections, microbiology diagnostics


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