| Title: | The influence of royal jelly and human interferon-alpha (HuIFN-[alpha]N3) on proliferation, glutathione level and lipid peroxidation in human colorectal adenocarcinoma cells in vitro |
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| Authors: | ID Filipič, Bratko (Author)
ID Gradišnik, Lidija (Author)
ID Rihar, Klemen (Author)
ID Šooš, Eugen (Author)
ID Pereyra Gonzales, Adriana (Author)
ID Potokar, Jana (Author) |
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| Files: |  Archives_of_Industrial_Hygiene_and_Toxicology_2015_Filipic_et_al._The_influence_of_royal_jelly_and_human_interferon-alpha_(HuIFN-αN3)_on.pdf (355,56 KB)
MD5: 258EF7A90FB5705D9AC81CF57414468B
 http://www.degruyter.com/view/j/aiht.2015.66.issue-4/aiht-2015-66-2632/aiht-2015-66-2632.xml
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | MF - Faculty of Medicine
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| Abstract: | Among royal jelly’s (RJ) various biological activities, its possible antitumour activity deserves particular attention. The purpose of this study was to investigate the influence of RJ, its bioactive component 10-hydroxy-2-decenoic acid (10- HDA), and human interferon-alpha (HuIFN-αN3) on the proliferation of human colorectal adenocarcinoma cells (CaCo- 2), and ascertain their effect on intracellular glutathione (GSH) level and lipid peroxidation. We studied the antiproliferative (AP) activity of RJ [(0.1 g/10 mL phosphate buffer saline (PBS)], HuIFN-αN3 (1000 I.U. mL-1), 10-HDA at 100.0 μmol L-1, and their different combinations, in the ratio 1:1, 1:2, and 2:1 on CaCo-2 cells. The GSH level was measured by glutathione assay. The lipid peroxidation was measured by malondialdehyde (MDA) assay. Single RJ had a low AP activity: 2.0 (0.5 mg mL-1). HuIFN-αN3 had an AP activity of 2.5 (208.33 I.U. mL-1), while 10-HDA had an AP activity of 1.5 (37.5 μmol mL-1). The highest AP activity of 3.8 was obtained when RJ and HuIFN-αN3 were applied at the ratio 2:1. In that combination the level of GSH was 24.9±2.4 nmol g-3 of proteins (vs. 70.2±3.2 nmol g-3 in the control) and the level of MDA was 72.3±3.1 nmol g-3 (vs. 23.6±9.1 nmol g-3 in the control). It is generally assumed that 10-HDA, an important constituent of RJ, together with HuIFN-αN3, is responsible for the inhibition of CaCo-2 cells proliferation in vitro. In our study, however, RJ and HuIFN-αN3 applied at 2:1 decreased the level of GSH the most and significantly increased lipid peroxidation via MDA in CaCo-2 cells. Future studies should show whether these GSH- and MDA-related activities of RJ, HuIFN-αN3, 10-HDA, and their combinations may decrease the tumorigenicity index and tumorigenic potential of various tumour cells in vitro. |
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| Keywords: | antiproliferative activity, antitumour activity, malondialddehyde, CaCo-2 cells, 10-hydroxy-2-decenoic acid |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Year of publishing: | 2015 |
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| Number of pages: | str. 269-274 |
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| Numbering: | Letn. 66, št. 4 |
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| PID: | 20.500.12556/DKUM-65318  |
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| ISSN: | 0004-1254 |
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| UDC: | 579 |
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| ISSN on article: | 0004-1254 |
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| COBISS.SI-ID: | 32433881 |
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| DOI: | 10.1515/aiht-2015-66-2632 |
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| NUK URN: | URN:SI:UM:DK:KYB4SCJD |
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| Publication date in DKUM: | 30.03.2017 |
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| Views: | 1309 |
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| Downloads: | 229 |
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| Metadata: |    |
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| Categories: | Misc.
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