| Title: | Effect of base sequence on G-wire formation in solution |
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| Authors: | ID Spindler, Lea (Author)
ID Rigler, Martin (Author)
ID Drevenšek Olenik, Irena (Author)
ID Webba da Silva, Mateus (Author)
ID Hessari, Nason Ma'ani (Author) |
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| Files: |  Journal_of_Nucleic_Acids_2010_Spindler_et_al._Effect_of_Base_Sequence_on_G-Wire_Formation_in_Solution.pdf (3,34 MB)
MD5: 82E99D3677ABF3EDAAAC176691B19100
 http://www.hindawi.com/journals/jna/2010/431651/
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FS - Faculty of Mechanical Engineering
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| Abstract: | The formation and dimensions of G-wires by different short G-rich DNA sequences in solution were investigated by dynamic light scattering (DLS) and polyacrilamide gel electrophoresis (PAGE). To explore the basic principles of wire formation, we studied the effects of base sequence, method of preparation, temperature, and oligonucleotide concentration. Both DLS and PAGE show that thermal annealing induces much less macromolecular self-assembly than dialysis. The degree of assembly and consequently length of G-wires (5-6 nm) are well resolved by both methods for DNA sequences with intermediate length, while some discrepancies appear for the shortest and longest sequences. As expected, the longest DNA sequence gives the longest macromolecular aggregates with a length of about 11 nm as estimated by DLS. The quadruplex topologies show no concentration dependence in the investigated DNA concentration range (0.1 mM–0.4 mM) and no structural change upon heating. |
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| Keywords: | DNA, dynamic light scattering, DLS, polyacrylamide gel electrophoresis, PAGE, genetics |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Year of publishing: | 2010 |
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| Number of pages: | str. 1-8 |
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| Numbering: | Letn. 2010 |
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| PID: | 20.500.12556/DKUM-66201  |
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| ISSN: | 2090-021X |
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| UDC: | 53 |
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| ISSN on article: | 2090-021X |
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| COBISS.SI-ID: | 23737127 |
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| DOI: | 10.4061/2010/431651 |
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| NUK URN: | URN:SI:UM:DK:TBKSDBHN |
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| Publication date in DKUM: | 14.06.2017 |
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| Views: | 2033 |
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| Downloads: | 393 |
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| Metadata: |    |
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| Categories: | Misc.
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