| Title: | Guanosine quadruplexes in solution : a small-angle X-ray scattering analysis of temperature effects on self-assembling of deoxyguanosine monophosphate |
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| Authors: | ID Mariani, Paolo (Author)
ID Spinozzi, Francesco (Author)
ID Federiconi, Francesco (Author)
ID Ortore, M. G. (Author)
ID Amenitsch, Heinz (Author)
ID Spindler, Lea (Author)
ID Drevenšek Olenik, Irena (Author) |
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| Files: |  Journal_of_Nucleic_Acids_2010_Mariani_et_al._Guanosine_Quadruplexes_in_Solution_A_Small-Angle_X-Ray_Scattering_Analysis_of_Temperature_E.pdf (4,23 MB)
MD5: AAFB1759E10519F5E2533BEE59018657
 http://www.hindawi.com/journals/jna/2010/472478/
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | FS - Faculty of Mechanical Engineering
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| Abstract: | We investigated quadruplex formation in aqueous solutions of -deoxyriboguanosine -monophosphate, d(pG), which takes place in the absence of the covalent axial backbone. A series of in-solution small angle X-ray scattering experiments on d(pG) have been performed as a function of temperature in the absence of excess salt, at a concentration just above the critical one at which self-assembling occurs. A global fit approach has been used to derive composition and size distribution of the scattering particles as a function of temperature. The obtained results give thermodynamical justification for the observed phase-behavior, indicating that octamer formation is essential for quadruplex elongation. Our investigation shows that d(pG) quadruplexes are very suitable to assess the potential of G-quadruplex formation and to study the self-assembling thermodynamics. |
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| Keywords: | G-quadruplexes, guanosine |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Year of publishing: | 2010 |
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| Number of pages: | str. 1-10 |
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| Numbering: | Letn. 2010 |
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| PID: | 20.500.12556/DKUM-66202  |
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| ISSN: | 2090-021X |
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| UDC: | 53 |
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| ISSN on article: | 2090-021X |
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| COBISS.SI-ID: | 23736871 |
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| DOI: | 10.4061/2010/472478 |
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| NUK URN: | URN:SI:UM:DK:53AFWAE0 |
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| Publication date in DKUM: | 14.06.2017 |
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| Views: | 1035 |
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| Downloads: | 227 |
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| Metadata: |    |
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| Categories: | Misc.
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