| Title: | A co-culture model of the developing small intestine offers new insight in the early immunomodulation of enterocytes and macrophages by Lactobacillus spp. through STAT1 and NF-kB p65 translocation |
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| Authors: | ID Trapečar, Martin (Author)
ID Goropevšek, Aleš (Author)
ID Gorenjak, Mario (Author)
ID Gradišnik, Lidija (Author)
ID Rupnik, Marjan (Author) |
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| Files: |  PLoS_ONE_2014_Trapecar_et_al._A_Co-Culture_Model_of_the_Developing_Small_Intestine_Offers_New_Insight_in_the_Early_Immunomodulation_of_E.PDF (2,86 MB)
MD5: F53EB2983D397E8F292C7D208E876F5B
 http://dx.plos.org/10.1371/journal.pone.0086297
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | MF - Faculty of Medicine
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| Abstract: | The early establishment of a complete microbiome has been shown to play an integral part in the development and maintenance of an intact intestine and its immune system, although much remains unknown about the specific mechanisms of immune modulation in newborns. In our study we show in a co-culture model of the undeveloped small intestine that members of Lactobacillus spp. influence STAT1 and NF-kB p65 nuclear translocation in both intestinal epithelial cells as well as underlying macrophages. Moreover, by using imaging flow cytometry we were able to monitor each individual cell and create a framework of the percentage of cells in which translocation occurred in challenged versus control cell populations. We also observed a significant difference in baseline translocation in intestinal cells when cultured alone versus those in a co-culture model, underpinning the importance of 3D models over monolayer set-ups in epithelial in vitro research. In conclusion, our work offers new insights into the potential routes by which the commensal microbiome primes the early immune system to fight pathogens, and shows how strain-specific these mechanisms really are. |
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| Keywords: | microbiome, Lactobacillus, immune system, pathogens |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Year of publishing: | 2014 |
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| Number of pages: | str. 1-8 |
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| Numbering: | Letn. 9, št. 1 |
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| PID: | 20.500.12556/DKUM-66266  |
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| ISSN: | 1932-6203 |
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| UDC: | 579.864 |
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| ISSN on article: | 1932-6203 |
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| COBISS.SI-ID: | 512370232 |
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| DOI: | 10.1371/journal.pone.0086297 |
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| NUK URN: | URN:SI:UM:DK:AZJFRQRO |
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| Publication date in DKUM: | 19.06.2017 |
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| Views: | 1638 |
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| Downloads: | 384 |
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| Metadata: |    |
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| Categories: | Misc.
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