| Title: | Malpighian tubule cells in overwintering cave crickets Troglophilus cavicola (Kollar, 1833) and T. neglectus Krauss, 1879 (Rhaphidophoridae, Ensifera) |
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| Authors: | ID Lipovšek Delakorda, Saška (Author)
ID Novak, Tone (Author)
ID Janžekovič, Franc (Author)
ID Weiland, Nina (Author)
ID Leitinger, Gerd (Author) |
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| Files: |  PLOS_ONE_2016_Lipovsek_et_al._Malpighian_Tubule_Cells_in_Overwintering_Cave_Crickets_Troglophilus_cavicola_(Kollar,_1833)_and_T._neglect.PDF (9,29 MB)
MD5: ECA237ED014A959CDF340FDFF9E31401
 http://dx.plos.org/10.1371/journal.pone.0158598
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| Language: | English |
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| Work type: | Scientific work |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: | MF - Faculty of Medicine
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| Abstract: | During winter, cave cricket larvae undergo dormancy in subterranean habitats; this dormancy is termed diapause in second year Troglophilus cavicola larvae because they mature during this time, and termed quiescence in T. neglectus, because they mature after dormancy. Here we used electron microscopy to analyze ultrastructural changes in the epithelial cells in the Malpighian tubules (MTs) of T. cavicola during diapause, in order to compare them with previous findings on T. neglectus. Moreover, the autophagosomes were studied with immunofluorescence microscopy in both species. Although the basic ultrastructure of the cells was similar, specific differences appeared during overwintering. During this natural starvation period, the nucleus, rER, the Golgi apparatus and mitochondria did not show structural changes, and the spherites were exploited. The abundances of autophagic structures in both species increased during overwintering. At the beginning of overwintering, in both species and sexes, the rates of cells with autophagic structures (phagophores, autophagosomes, autolysosomes and residual bodies) were low, while their rates increased gradually towards the end of overwintering. Between sexes, in T. cavicola significant differences were found in the autophagosome abundances in the middle and at the end, and in T. neglectus at the end of overwintering. Females showed higher rates of autophagic cells than males, and these were more abundant in T. cavicola. Thus, autophagic processes in the MT epithelial cells induced by starvation are mostly parallel in diapausing T. cavicola and quiescent T. neglectus, but more intensive in diapausing females. |
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| Keywords: | cave cricket, overwintering, Troglophilus cavicola, Troglophilus neglectus |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Year of publishing: | 2016 |
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| Number of pages: | str. 1-16 |
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| Numbering: | Letn. 11, št. 7 |
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| PID: | 20.500.12556/DKUM-66282  |
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| ISSN: | 1932-6203 |
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| UDC: | 591.1 |
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| ISSN on article: | 1932-6203 |
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| COBISS.SI-ID: | 22340104 |
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| DOI: | 10.1371/journal.pone.0158598 |
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| NUK URN: | URN:SI:UM:DK:NKEWZ9P2 |
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| Publication date in DKUM: | 19.06.2017 |
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| Views: | 1605 |
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| Downloads: | 398 |
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| Metadata: |    |
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| Categories: | Misc.
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