| Naslov: | The relationship between membrane potential and calcium dynamics in glucose-stimulated beta cell syncytium in acute mouse pancreas tissue slices |
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| Avtorji: | ID Dolenšek, Jurij (Avtor)
ID Stožer, Andraž (Avtor)
ID Skelin, Maša (Avtor)
ID Miller, Evan (Avtor)
ID Rupnik, Marjan (Avtor) |
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| Datoteke: |  PLoS_ONE_2013_Dolensek_et_al._The_Relationship_between_Membrane_Potential_and_Calcium_Dynamics_in_Glucose-Stimulated_Beta_Cell_Syncytium.PDF (2,57 MB)
MD5: 8BEB97CED03AC58BDFE7F24E9DE6BF0F
 http://dx.plos.org/10.1371/journal.pone.0082374
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Znanstveno delo |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | MF - Medicinska fakulteta
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| Opis: | Oscillatory electrical activity is regarded as a hallmark of the pancreatic beta cell glucose-dependent excitability pattern. Electrophysiologically recorded membrane potential oscillations in beta cells are associated with in-phase oscillatory cytosolic calcium activity ([Ca2+]i) measured with fluorescent probes. Recent high spatial and temporal resolution confocal imaging revealed that glucose stimulation of beta cells in intact islets within acute tissue slices produces a [Ca2+]i change with initial transient phase followed by a plateau phase with highly synchronized [Ca2+]i oscillations. Here, we aimed to correlate the plateau [Ca2+]i oscillations with the oscillations of membrane potential using patch-clamp and for the first time high resolution voltage-sensitive dye based confocal imaging. Our results demonstrated that the glucose-evoked membrane potential oscillations spread over the islet in a wave-like manner, their durations and wave velocities being comparable to the ones for [Ca2+]i oscillations and waves. High temporal resolution simultaneous records of membrane potential and [Ca2+]i confirmed tight but nevertheless limited coupling of the two processes, with membrane depolarization preceding the [Ca2+]i increase. The potassium channel blocker tetraethylammonium increased the velocity at which oscillations advanced over the islet by several-fold while, at the same time, emphasized differences in kinetics of the membrane potential and the [Ca2+]i. The combination of both imaging techniques provides a powerful tool that will help us attain deeper knowledge of the beta cell network. |
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| Ključne besede: | glucose, pancreas, mice |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Leto izida: | 2013 |
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| Št. strani: | str. 1-16 |
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| Številčenje: | Letn. 8, št. 12 |
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| PID: | 20.500.12556/DKUM-66303  |
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| ISSN: | 1932-6203 |
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| UDK: | 612.349.7 |
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| COBISS.SI-ID: | 512362552 |
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| DOI: | 10.1371/journal.pone.0082374 |
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| ISSN pri članku: | 1932-6203 |
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| NUK URN: | URN:SI:UM:DK:H6SGPS3R |
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| Datum objave v DKUM: | 19.06.2017 |
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| Število ogledov: | 1483 |
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| Število prenosov: | 386 |
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| Metapodatki: |    |
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| Področja: | Ostalo
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