| Naslov: | Use of non-conventional cell disruption method for extraction of proteins from black yeasts |
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| Avtorji: | ID Čolnik, Maja (Avtor)
ID Primožič, Mateja (Avtor)
ID Knez, Željko (Avtor)
ID Leitgeb, Maja (Avtor) |
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| Datoteke: |  Frontiers_in_Bioengineering_and_Biotechnology_2016_Colnik_et_al._Use_of_Non-Conventional_Cell_Disruption_Method_for_Extraction_of_Protei.pdf (2,13 MB)
MD5: 4C3C9BE60F6C16F194250FD079612AD4
PID: 20.500.12556/dkum/f22c92d9-01b8-4787-9a70-67f64f61e592
 http://journal.frontiersin.org/article/10.3389/fbioe.2016.00033/full
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| Jezik: | Angleški jezik |
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| Vrsta gradiva: | Znanstveno delo |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: | FKKT - Fakulteta za kemijo in kemijsko tehnologijo
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| Opis: | The influence of pressure and treatment time on cells disruption of different black yeasts and on activities of extracted proteins using supercritical carbon dioxide process was studied. The cells of three different black yeasts Phaeotheca triangularis, Trimatostroma salinum, and Wallemia ichthyophaga were exposed to supercritical carbon dioxide (SC $CO_2$) by varying pressure at fixed temperature (35°C). The black yeasts cell walls were disrupted, and the content of the cells was spilled into the liquid medium. The impact of SC $CO_2$ conditions on secretion of enzymes and proteins from black yeast cells suspension was studied. The residual activity of the enzymes cellulase, $beta$-glucosidase, $alpha$-amylase, and protease was studied by enzymatic assay. The viability of black yeast cells was determined by measuring the optical density of the cell suspension at 600 nm. The total protein concentration in the suspension was determined on UV–Vis spectrophotometer at 595 nm. The release of intracellular and extracellular products from black yeast cells was achieved. Also, the observation by an environmental scanning electron microscopy shows major morphological changes with SC $CO_2$-treated cells. The advantages of the proposed method are in a simple use, which is also possible for heat-sensitive materials on one hand and on the other hand integration of the extraction of enzymes and their use in biocatalytical reactions. |
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| Ključne besede: | P. triangularis, W. ichtyophaga, T. salinum, supercritical carbon dioxide, enzyme activity, cells |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Leto izida: | 2016 |
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| Št. strani: | str. 1-12 |
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| Številčenje: | Letn. 4 |
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| PID: | 20.500.12556/DKUM-66788  |
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| ISSN: | 2296-4185 |
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| UDK: | 66 |
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| COBISS.SI-ID: | 19560726 |
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| DOI: | 10.3389/fbioe.2016.00033 |
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| ISSN pri članku: | 2296-4185 |
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| NUK URN: | URN:SI:UM:DK:6BTSLNMZ |
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| Datum objave v DKUM: | 10.07.2017 |
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| Število ogledov: | 1738 |
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| Število prenosov: | 416 |
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| Metapodatki: |    |
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| Področja: | Ostalo
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