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Title:Pridobivanje transglutaminaze iz bakterije streptomyces platensis
Authors:ID Lang, Vida (Author)
ID Leitgeb, Maja (Mentor) More about this mentor... New window
ID Čolnik, Maja (Comentor)
Files:.pdf MAG_Lang_Vida_2017.pdf (3,08 MB)
MD5: D940DAB84C4B31AFBB852CF61DCBEB7F
PID: 20.500.12556/dkum/42169701-6345-4171-90eb-5eaead30212e
 
Language:Slovenian
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FNM - Faculty of Natural Sciences and Mathematics
Abstract:Streptomyces platensis je bakterija iz rodu Streptomyces. V rod Streptomyces spadajo nitaste bakterije, ki jih najdemo v prsti. Bakterije Streptomyces uspevajo pri pH 6,5 – 8,0 in v temperaturnem območju med 25 °C in 35 °C. Za S. platensis je značilna proizvodnja zunajceličnih encimov, ki se uporabljajo za industrijske namene. Namen magistrske naloge je bil ugotoviti kako sprememba pH v celični suspenziji S. platensis vpliva na maso celic S. platensis, aktivnost encima transglutaminaza in koncentracijo skupnih proteinov v bakterijski suspenziji S. platensis. Celično suspenzijo S. platensis smo inkubirali v inkubacijskem stresalniku 8 dni pri temperaturi 28 °C in ob rahlem stalnem stresanju. Naredili smo 3 izvedbe, pri katerih smo v procesu inkubiranja celične suspenzije S. platensis spreminjali pogoje pH. Pri vseh 3 izvedbah inkubiranja celične suspenzije S. platensis v odvisnosti od inkubacijskega časa smo ugotovili, da medtem ko se je pH celične suspenzije zniževal, so koncentracija proteinov, aktivnost transglutaminaze in prirast suhe mase celic naraščale. Po treh različnih izvedbah se je izkazalo, da je za gojenje celične suspenzije S. platensis najugodnejši začetni pH 6,7 in inkubiranje pri temperaturi 28 °C ob rahlem stresanju ter brez dodatnega spreminjanja pH. Spreminjanje oziroma uravnavanje pH na začetku procesa ali med celotnim procesom inkubiranja celične suspenzije S. platensis ni ugodno za uspešno gojenje bakterije S. platensis, saj se celoten proces pospeši in bakterije hitreje propadejo.
Keywords:S. platensis, aktivnost transglutaminaze, koncentracija proteinov, pH, masa celic.
Place of publishing:Maribor
Publisher:[V. Lang]
Year of publishing:2017
PID:20.500.12556/DKUM-67178 New window
UDC:543.645.4:579.873.7(497.411)(043.2)
COBISS.SI-ID:23361288 New window
NUK URN:URN:SI:UM:DK:YDADDFEQ
Publication date in DKUM:22.09.2017
Views:2641
Downloads:248
Metadata:XML DC-XML DC-RDF
Categories:FNM
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Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:04.08.2017

Secondary language

Language:English
Title:Extraction of transglutaminase from the bacterium streptomyces platensis
Abstract:Streptomyces platensis is bacteria from the genus of Streptomyces. Part of the genus Streptomyces are thread-like bacteria, which are found in the soil. Bacteria Streptomyces thrive at pH 6,5 – 8,0 and in the temperature range between 25 and 35 °C. S. platensis are known for the production of extracellular enzymes, which are being used for industrial purposes. The purpose of this master thesis was to find out how changes of pH in the cellular suspension of S. platensis affect the mass of cells of S. platensis, how they affect the activity of transglutaminase enzyme and how they affect the concentration of total proteins in bacterial suspension. We have incubated cellular suspension of S. platensis in an incubator shaker for 8 days at a temperature of 28 °C and constant mild shaking. We have performed three trials, during which we have been changing pH conditions in the process of incubation of cellular suspension of S. platensis. At all three trials of incubation of the cellular suspension of S. platensis we have discovered in respect to the incubating time that while the pH of the cellular suspension was dropping, the concentration of proteins, the activity of transglutaminase and the growth of the dry mass of cells were rising. After three different trials it has turned out, that for the growth of the cellular suspension of S. platensis it is ideal to have a starting value of pH to be 6,7 and for incubating to occur at 28 °C with constant mild shaking and without additional altering of the pH value. Changing the value of pH either at the start or during the process of incubation of the cellular suspension of S. platensis is not beneficial for the successful growth of the bacteria S. platensis as the entire process is being accelerated and bacteria decay faster.
Keywords:S. platensis, activity of transglutaminase, concentration of proteins, pH, cellular mass.


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