Preučevanje koloidnih lastnosti magnetnih nanodelcev prevlečenih s polipeptidom

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Title:	Preučevanje koloidnih lastnosti magnetnih nanodelcev prevlečenih s polipeptidom
Authors:	ID Rešeta, Saša (Author) ID Gyergyek, Sašo (Mentor) More about this mentor... ID Pahovnik, David (Comentor)
Files:	MAG_Reseta_Sasa_2017.pdf (2,75 MB) MD5: 39425C00F7B59692CF0F980C8E440F6E PID: 20.500.12556/dkum/20a458b2-b2d5-4d35-8f00-1b38308c715f
Language:	Slovenian
Work type:	Master's thesis/paper
Typology:	2.09 - Master's Thesis
Organization:	FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:	Stabilne suspenzije magnetnih nanodelcev prevlečenih z bioaktivnimi molekulami predstavljajo velik potencial v terapevtskih aplikacijah. Goli magnetni nanodelci v vodnih suspenzijah imajo težnjo po aglomeraciji, z adsorpcijo aminokislin nanje pa lahko vplivamo na njihov površinski naboj in tako pripravimo stabilne suspenzije. V magistrskem delu smo želeli pripraviti stabilne suspenzije magnetnih nanodelcev maghemita prevlečenih s polipeptidom na enostaven in cenovno ugoden način, primeren za masovno industrijsko proizvodnjo. Preučevali smo tudi obstojnost peptidne prevleke v biološko pomembnem mediju, fosfatnem pufru, ki ima veliko afiniteto do površine nanodelca. Magnetne nanodelce smo sintetizirali s soobarjanjem Fe2+/Fe3+ ionov iz vodne raztopine. Na tako pripravljene nanodelce smo adsorbirali aminokislini lizin in asparaginsko kislino v različnih razmerjih. Za zamreženje adsorbiranih aminokislin smo uporabili zamreževalni reagent 1-etil-3-(-3-dimetilaminopropil) karbodiimid hidroklorid (EDC), ki je znan po nizki toksičnosti in sposobnosti modifikacije stranskih skupin proteinov. Z adsorpcijo aminokislin na površino magnetnih nanodelcev in naknadnim dodatkom zamreževalnega reagenta nismo uspeli pripraviti koloidno stabilnih suspenzij. S FT-IR meritvami smo potrdili nastanek peptida na površini magnetnih nanodelcev. Na podlagi HPLC meritev aminokislin v supernatantih suspenzij nanodelcev sklepamo, da se na nanodelce v prisotnosti EDC adsorbira precejšen del aminokislin, pri čemer se asparaginska kislina adsorbira v večji meri kot lizin. Meritve zeta potenciala so pokazale obstojnost peptidne prevleke na nanodelcih v prisotnosti fosfatnega pufra s koncentracijo, ki se uporablja v bioloških aplikacijah.
Keywords:	magnetni nanodelci, maghemit, asparaginska kislina, lizin, EDC, PBS
Place of publishing:	Maribor
Publisher:	[S. Rešeta]
Year of publishing:	2017
PID:	20.500.12556/DKUM-68196
UDC:	620.3:665.7.035.2(043.2)
COBISS.SI-ID:	21030422
NUK URN:	URN:SI:UM:DK:WRPD7VIO
Publication date in DKUM:	22.11.2017
Views:	1961
Downloads:	197
Metadata:
Categories:	KTFMB - FKKT
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Licences

License:	CC BY 4.0, Creative Commons Attribution 4.0 International

Link:	http://creativecommons.org/licenses/by/4.0/
Description:	This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:	12.09.2017

Secondary language

Language:	English
Title:	Colloidal properties of magnetic nanoparticles coated by polypeptides
Abstract:	Stable suspensions of magnetic nanoparticles coated with bioactive molecules show great potential for therapeutic applications. Magnetic nanoparticles in aqueous suspensions have the tendency to agglomerate, and with their adsorption of amino acids we can influence their surface charge, thus preparing stable suspensions. In this Master’s thesis, we aimed to prepare stable suspensions of maghemite magnetic nanoparticles coated with a polypeptide in a simple and cost-effective manner suitable for mass industrial production. We also researched the stability of a peptide coating in a biologically important medium, a phosphate buffer, which has a great affinity for the surface of the nanoparticle. The magnetic nanoparticles were synthesized through the co-precipitation of the Fe2+/Fe3+ ions in the aqueous solution. Once the nanoparticles were prepared, we adsorbed the amino acids lysine and aspartic acid in varying proportions. In order to cross-link the adsorbed amino acids we used the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) cross-linking reagent, which is known for its low toxicity and its ability to modify side groups of proteins. With the adsorption of the amino acids onto the surface of the magnetic nanoparticles and the subsequent addition of the cross-linking reagent, we were unable to prepare colloidally stable suspensions. FT-IR measurements confirmed the formation of a peptide on the surface of magnetic nanoparticles. Based on our HPLC measurements of amino acids in supernatant nanoparticle suspensions, we can conclude that in the presence of EDC nanoparticles adsorb a substantial portion of the amino acids, of which aspartic acid is adsorbed to a greater extent than lysine. Our measurements of the zeta potential demonstrated the persistence of the peptide coating on the nanoparticles in the presence of a phosphate buffer at a concentration used in biological applications.
Keywords:	magnetic nanoparticles, maghemite, aspartic acid, lysine, EDC, PBS

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