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Title:Študija aktivnosti nekaterih encimov v gelu aloe vere
Authors:ID Bandur, Patricija (Author)
ID Leitgeb, Maja (Mentor) More about this mentor... New window
ID Vasić, Katja (Comentor)
Files:.pdf UN_Bandur_Patricija_2018.pdf (2,21 MB)
MD5: 6A6F489431008423668119CF80AA8B5F
PID: 20.500.12556/dkum/a20b8c8e-c736-4c99-91a4-d97dab74b360
 
Language:Slovenian
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:Aloja, rastlina nesmrtnosti, kot jo nekateri poimenujejo, je v današnjem času izredno priljubljena, predvsem zaradi svojih zdravilnih učinkov. Prihaja iz družine zlatokorenovk. Poznamo več kot 200 vrst aloje, vendar se v zdravilne namene uporabljata samo dve, to sta Aloe arborescens in Aloe barbadensis, ki jo imenujemo tudi Aloe vera. Aloja je sestavljena iz 99 % vode, ostali 1 % pa predstavljajo zdravilne učinkovine, kot so vitamini, encimi, sladkorji, minerali, saponini, lignin, salicilna kislina in aminokisline. V diplomski nalogi bomo podrobneje opisali encime, ki jih vsebuje aloja, kot tudi določili njihove vsebnosti v hladilnem gelu, ki je v zadnjem času izredno priljubljen pri celjenju ran. V okviru diplomskega dela smo pripravili lasten gel iz aloje in primerjali encimsko aktivnost med kupljenim in domačim gelom. Encimi, ki jih vsebuje aloja, so amilaza, bradikinaza, karboksipeptidaza, katalaza, celulaza, kreatin-foskinaza, fosfataza, lipaza, proteaza, transminaza in tirozinaza. Odločili smo se, da bomo preverili aktivnosti naslednjih encimov: celulaze, α-amilaze, proteaze, peroksidaze, katalaze, transglutaminaze in lipoproteinske lipaze. Opravili smo encimske teste, izmerili absorbanco na UV/Vis-spektrofotometru v pripravljenih vzorcih ter s pomočjo izmerjene absorbance določili aktivnost encimov v A. arborescens in A. barbadensis. Aktivnosti encimov so bile nizke zaradi velikega deleža vode v aloji.
Keywords:A. arborescens, A. barbadensis, proteini, encimi.
Place of publishing:Maribor
Publisher:[P. Bandur]
Year of publishing:2018
PID:20.500.12556/DKUM-71589 New window
UDC:543.645.4:543.384(043.2)
COBISS.SI-ID:21935382 New window
NUK URN:URN:SI:UM:DK:I1QPC5Z1
Publication date in DKUM:09.10.2018
Views:2139
Downloads:293
Metadata:XML DC-XML DC-RDF
Categories:KTFMB - FKKT
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Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:22.08.2018

Secondary language

Language:English
Title:Study of the activity of certain enzymes in the gel of aloa vera
Abstract:Aloe or the plant of immortality as some would call it, is very popular nowadays, mainly because of its healing effects. It originates from the family of Asphodelaceae. There is a lot of species of Aloja, over 200 of them, but only 2 are used for its healing effects, and those are A.arborescens and A. barbadesis which is more known as Aloe vera. Aloe is composed from 99% of water and the remaining 1% are active substances such as vitamins, enzymes, sugars, minerals, saponins, lignin, salicylic acid and amino acids. In this graduation thesis we will mainly focus on enzymes that aloe consists of and also the presence of enzymes in cooling gel, which is very popular lately for its healing properties for different wounds. We therefore decided to make our homemade aloe cooling gel and then compare its enzyme activity to the one bought in the store. Enzymes that aloe contains of are amylase, bradykinase, carboxypeptidase, catalase, cellulase, creatine phoskinase, phosphatase, lipase, protease, transminosis and tyrosinase. We decided to determine the activity of the following enzymes: cellulase, α-amylase, protease, horseradish peroxidase, catalase, transglutaminase, and lipoprotein lipase. Some tests, measurements were performed and the absorbance on the UV-Vis spectrophotometre was measured, which helped us to determine the activity of enzymes. The activites were very low, due to large amount of water in Aloe.
Keywords:A. arborescens, A. barbadensis, proteins, enzymes


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