Novi kompoziti nanodelcev Fe2O3  in polimernih nosilnih materialov- razširjanje možnosti biomedicinske uporabe

Rajh, Tina

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Title:	Novi kompoziti nanodelcev Fe2O3 in polimernih nosilnih materialov- razširjanje možnosti biomedicinske uporabe
Authors:	ID Rajh, Tina (Author) ID Kristl, Matjaž (Mentor) More about this mentor... ID Maver, Uroš (Comentor) ID Ban, Irena (Comentor)
Files:	MAG_Rajh_Tina_2019.pdf (2,23 MB) MD5: 705C7818E08A1CA2604EDDA7447BD325 PID: 20.500.12556/dkum/f3ea2f78-4b04-465b-944f-1f00c08a4cee
Language:	Slovenian
Work type:	Master's thesis/paper
Typology:	2.09 - Master's Thesis
Organization:	FKKT - Faculty of Chemistry and Chemical Engineering
Abstract:	Namen magistrske naloge je priprava biokompatibilnih nanodelcev Fe2O3 s primerno magnetizacijo in Curiejevo temperaturo (Tc). V prvi fazi smo sintetizirati magnetne Fe2O3 nanodelce s pomočjo soobarjanja in jih nato oblekli še s siliko. Nato smo s pomočjo rentgenske praškovne difrakcije (XRD) ter z metodo Fourierjeve transformirane infrardeče spektroskopije (FTIR) analizirali kemijsko sestavo sintetiziranih nanodelcev ter kasneje z metodo dinamičnega sipanja svetlobe (DLS) in z uporabo transmisijskega elektronskega mikroskopa (TEM) preverili ali so delci oblečeni s siliko ter tako primerni za nadaljne delo. S termogravimetrično metodo (TGA) smo določili Curiejevo temperaturo (Tc) ter opazovali kako se spreminja masa delcev s časom po termičnem razkroju. V naslednji fazi smo pripravljali tanke filme na osnovi alginata z vgrajenimi nanodelci Fe2O3 in modelne zdravilne učinkovine (ZU) lidokaina (LID), pogosto uporabljen lokalni anestetik. Tanke filme smo pripravili na dva različna načina in sicer na:  In situ način: nanodelce smo prelili z raztopino učinkovine v etanolu.  Ločeno vgrajevanje nanodelcev in učinkovine lidokaina v tanke filme. Tanke filme smo pripravili v treh slojih s pomočjo »spin- coating« metode. Nato smo s pomočjo UV-VIS spektrofotometra spremljali sproščanje zdravilne učinkovine (ZU) lidokaina. Sproščanje je potekalo 24 ur v Francovih difuzijskih celicah. Dokazali smo, da je sproščanje zdravilne učinkovine bilo uspešno ter da s koncentracijo vgrajene zdravilne učinkovine v tanke filme lahko vplivamo tudi na sproščanje zdravilne učinkovine. S pomočjo uporabljenih metod smo dokazali, da so pripravljeni delci primerni za nadaljnje preskušanje v smeri biomedicinskih aplikacij, še posebej na področju zdravljenja raka ali kot kontrastna sredstva.
Keywords:	nanodelci, Fe2O3, soobarjanje, lidokain, alginat, Curiejeva temperatura
Place of publishing:	Maribor
Publisher:	[T. Rajh]
Year of publishing:	2018
PID:	20.500.12556/DKUM-73078
UDC:	544.023.22:615.015.1(043.2)
COBISS.SI-ID:	22194966
NUK URN:	URN:SI:UM:DK:C7HRRAES
Publication date in DKUM:	04.03.2019
Views:	1429
Downloads:	193
Metadata:
Categories:	KTFMB - FKKT
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Licences

License:	CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International

Link:	http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:	The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:	04.02.2019

Secondary language

Language:	English
Title:	New composites of Fe2O3 nanoparticles and polymer carrier materials for possible biomedical applications
Abstract:	The purpose of this master thesis is the preparation of biocompatible Fe2O3 nanoparticles with a suitable magnetization and Curie temperature (Tc). In the first phase we synthesized magnetic Fe2O3 nanoparticles with the help of a co-precipitate and coated these with silica. With the help of X-ray powder diffraction (XRD) and the Fourier transform infrared spectroscopy (FTIR) we analysed the chemical composition of the synthesized nanoparticles and later on, by using dynamic light scattering (DLS) and transmission electron microscopy (TEM), we checked if the particles are indeed coated with silica and therefore ready for further work. With the thermogravimetric analysis (TGA) we determined the Curie temperature (Tc) and observed the changes in mass of the particles after thermic disintegration. In the next phase, we prepared alginate-based thin films with incorporated Fe2O3 nanoparticles and the model drug lidocaine (LID), a common local anaesthetic. We prepared the thin films in two different ways:  In situ approach: we covered the nanoparticles with the solution of the active substance in ethanol.  Separate incorporation of nanoparticles and the active substance lidocaine into the thin films. The thin films were prepared in three layers with the help of the »spin-coating« method. With the help of UV-VIS spectrophotometry, we then followed the release of the active substance (AS) lidocaine. The release took 24 hours in the Franz diffusion cells. We proved, that the active substance was release successfully, and that the release of the active substance can be altered with the incorporated concentration of the active substance. With the help of the used methods we have shown that the as-prepared nanoparticles are suitable for further testing towards potential use in biomedicine. Among their most interesting potential applications are the use in cancer treatment or as contrast agents.
Keywords:	nanoparticles, Fe2O3, co-precipitation, lidocaine, alginate, Curie temperature

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